东亚飞蝗mariner转座子片段的克隆和序列分析  

Cloning and Analysis of mariner-like Element Fragment in Migratory Locust,Locusta migratoria manilensis (Meyen)

作　　者：谢妤[1] 何正波[1] 陈斌[1] 

机构地区：[1]重庆师范大学生命科学学院昆虫与分子生物学研究所,重庆400047

出　　处：《应用与环境生物学报》2010年第6期812-816,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(No.30700435);重庆市自然科学基金项目(No.2009BB1387)资助~~

摘　　要：mariner转座子在昆虫中广泛存在,转座时不需要宿主特异因子的作用,是昆虫种系转化的有力工具.以mariner转座酶的保守序列设计的简并引物从东亚飞蝗Locusta migratoria manilensis(Meyen)基因组中扩增出7个序列有差异的片段,其中的1个片段能够翻译成为连续的氨基酸序列,另外6个片段中均含有终止子或移码突变.经同源性比对、序列特征分析,证实东亚飞蝗基因组中存在mariner类元件,但大部分都是无功能的假基因;系统进化分析发现东亚飞蝗基因组中的mariner类元件有可能属于新的mariner次级家族.The mariner-like elements（MLEs） are widely distributed in insects.Owing to their wide distribution and the ability to move from one location to another without the help of specific host factors,MLEs become powerful tools for genetic manipulation of insect species with special emphasis on insects of economic importance.In that respect,the presence of MLEs was assessed in Locusta migratoria manilensis（Meyen） by polymerase chain reaction（PCR） using degenerate primers designed from two conserved regions of mariner transposase open reading frame.Seven PCR fragments with variation in sequence were identified from the locust,and among them one fragment showed a continuous amino acid sequence and the other 6 PCR fragments were pseudogenes with stop codons,frameshifts or deletions in the deduced amino acid sequences.Homologous analysis and sequence character showed that the fragment with continuous amino acid sequence was MLE and possibly belonged to a new subfamily of mariner family.These data suggested that L.migratoria manilensis possessed MLE,but mostly existed as pseudogenes in its genome.

关 键 词：东亚飞蝗 MARINER 转座子 克隆 进化分析 

分 类 号：S433[农业科学—农业昆虫与害虫防治] Q78[农业科学—植物保护]