高效液相色谱法测定复方斑蝥胶囊中野黄芩苷含量  

Determination of Scutellarin Content in Compound Banmao Capsules by HPLC

作　　者：戴薇[1] 

机构地区：[1]重庆医科大学附属第二医院,重庆400010

出　　处：《中国药业》2011年第1期23-24,共2页China Pharmaceuticals

摘　　要：目的建立测定复方斑蝥胶囊中野黄芩苷含量的高效液相色谱法。方法采用C18色谱柱(250 mm×4.6 mm,5μm),以甲醇-0.3%磷酸溶液(40∶60)为流动相,检测波长为335 nm。结果野黄芩苷进样量在0.122 76～1.227 6μg范围内与峰面积线性关系良好,加样回收率为98.61%,RSD为0.49%(n=6)。结论高效液相色谱法快速、准确,可用于复方斑蝥胶囊的质量控制。Objective To determine the content of scutellarin in Compound Banmao Capsules.Methods The HPLC method was adopted and the C18 column（250 mm×4.6 mm,5 μm） was used.The mobile phase was methanol-0.3%phosphoric acid（40 ∶60）.The detection wavelength was at 335 nm.Results The calibration curve of scutellarin was linear in the range of 0.122 76-1.227 6 μg（r=0.999 9）,the average recovery rate was 98.61%,RSD was 0.49%（n=6）.Conclusion The method is quick and accurate,which can be used for the quality control of Compound Banmao Capsules.

关 键 词：野黄芩苷 复方斑蝥胶囊 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]