肿瘤坏死因子α与白细胞介素6在糖尿病大鼠骨骼肌中表达的实验研究  

The expression of tumor necrosis factor-α and interleukin-6 in quadriceps femoris muscle of diabetic rats

作　　者：刘淑娟[1] 王君[1] 王志辉[1] 

机构地区：[1]山东省青岛市第八人民医院内分泌科,266100

出　　处：《中国医药》2011年第1期73-75,共3页China Medicine

基　　金：山东省青岛市科技发展指导计划项目（KZJ-29）

摘　　要：目的 探讨糖尿病大鼠骨骼肌中炎性细胞因子肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6的表达及其与细胞凋亡的关系.方法 将Wistar大鼠16只分为实验组和对照组各8只.实验组饲以8周高脂高糖饮食后腹腔内注射小剂量链脲佐菌素（30 mg/kg）.对照组仅注射柠檬酸-柠檬酸钠缓冲液.第13周末处死大鼠.观察TNF-α及IL-6的蛋白表达,检测骨骼肌细胞中caspase 3的表达,并计算二者的相关性.结果 与对照组数据[TND-a、IL-6、caspase 3分别为（0.63±0.35）%、（2.05±0.83）%、（10.02±0.82）%]相比,实验组大鼠骨骼肌中TNF-α与IL-6（3.75±1.38%,5.77±0.74%）的表达水平均升高（P＜0.01）,caspase 3[（10.02±0.82）%]表达明显高于（P＜0.01）,TNF-α、IL-6与caspase3的表达具有相关性（r=0.713,r=0.692,P＜0.05）.结论 糖尿病骨骼肌病变时炎症与凋亡同时发生,并可能共同导致胰岛素抵抗.Objective To evaluate changes of tumor necrosis factor-α （TNF-α） and interleukin-6 （IL-6）expression in quadriceps femoris muscle of diabetic and control rats, and to explore the corelation among TNF-α, IL-6 and caspase 3. Methods Totally 16 adult （aged 8 weeks） Wistar rats were divided into non-diahetic （n =8）group and diabetic （n = 8） group. Diabetic rats were fed by high lipid and glucose diet. After 8 weeks, they were made by a single injection of streptozotocin （STZ） at a dose of 30 mg/kg and control rats were injected by citrate buffer （ pH 4.5 ） in the same dose. Quantitative immunohistochemistry assay for TNF-α, IL-6 and caspase 3 was applied. The quantitative analysis was used to evaluate the positive rate of TNF-α, IL-6 and caspase 3 expression and correlation analysis was used to evaluate the corelation of them. Results TNF-α, IL-6 protein had higher expression （3.75 ± 1.38,5.77 ± 0.74） in quadriceps femoris muscle of diabetic rats than those in controls （P 〈 0.01 ）.Caspase 3 （ 10.02 ±0.82） had higher expression in muscle of diabetic rats than that in controls （P 〈0.01 ）. There was significant correlation among TNF-α, IL-6 and caspase 3 （r=0. 713,r=0. 692,P 〈0.05）. Conclusions In muscle of diabetic rats, apoptosis is closely related to TNF-α and IL-6 protein which may induce the insulin resistance.

关 键 词：糖尿病 肿瘤坏死因子Α 白细胞介素6 免疫组化 

分 类 号：R587.1[医药卫生—内分泌]