凉血通瘀方对脑出血大鼠脑组织t-PA和MMP-9表达的影响  

Effect of Liangxue Tongyu Formula on t-PA and MMP-9 expressions in rats with intracerebral hemorrhage

作　　者：何春燕[1] 黄建华[2] 周学平[3] 金妙文[3] 叶放[3] 吴海涛[4] 陈伟华[2] 吴勉华[3] 

机构地区：[1]南京中医药大学中医学博士后流动站,南京210029 [2]复旦大学中西医结合研究所,上海200040 [3]南京中医药大学第一临床医学院,南京210029 [4]南京中医药大学基础医学院,南京210029

出　　处：《中华中医药杂志》2011年第1期50-53,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重点基础研究发展计划973计划(No.2006CB504807);中国博士后科学基金资助项目(No.20080441068);南京中医药大学中医内科学重点学科资助项目(No.zynk006)~~

摘　　要：目的:观察中药凉血通瘀方对脑出血大鼠脑组织纤溶酶原激活物(t-PA)和基质金属蛋白酶9(MMP-9)表达的影响。方法:采用自体不凝血方法制备大鼠脑出血模型,分为模型组和凉血通瘀组和伪手术组。凉血通瘀组给予凉血通瘀煎剂、模型组和伪手术组给予生理盐水每日灌胃。脑出血后24、48、72、120h观测大鼠血肿含量、血脑屏障通透性,采用Western blot和荧光RT PCR检测血肿周围组织内t-PA和MMP-9蛋白和基因表达水平。结果:凉血通瘀组各时间点血肿含量显著低于模型组(P<0.01),血脑屏障通透性亦低于模型组(P<0.01)。与模型组相比,脑出血后48、72、120h,凉血通瘀组的t-PA蛋白和基因表达水平显著升高,而MMP-9水平显著降低(P<0.01)。结论:凉血通瘀方促进血肿吸收、改善血脑屏障通透性可能和其上调脑出血大鼠脑内t-PA表达、下调脑内MMP-9表达有关。Objective： To evaluate the effect of Liangxue Tongyu Formula on t-PA and MMP-9 expressions in rats with intercerebral hemorrhage.Methods： Intracerebral hemorrhage（ICH） was induced by using the intrastriatal autologous blood injection model.ICH rats were randomized into model group,Liangxue Tongyu group and sham-operated group.Rats in Liangxue Tongyu group were intragastrically administrated with Liangxue Tongyu decotion every day while the other two groups were given saline.Hematoma quantification,permeability of blood brain barrier at 24h,48h,72h,and 120h after ICH,were determined,respectively.t-PA and MMP-9 protein and gene expressions in perihematoma were detected by Western blot and RT PCR at the four time points,respectively.Results： Hematoma quantification at 48h,72h and 120h after ICH in Liangxue Tongyu group were lower than that of model group（P0.05,P0.01,P0.01,respectively）.Meanwhile,the permeability of blood brain barrier in Liangxue Tongyu group after ICH was lower than that of model group,and it turned to be normal at 120h.t-PA expression both in Liangxue Tongyu group and model group elevated at 72h,but the difference between model group and sham-operated group was not significant（P=0.056）.Compared with model group,Liangxue Tongyu group had higher protein and gene expression of t-PA at 48h,72h and 120h after ICH.However,MMP-9 levels in CHF group at the three time points were lower than those of model group,although MMP-9 expressed in a large quantity in both groups（vs sham-operated group,P0.01,respectively） after ICH.Conclusion： Liangxue Tongyu Formula facilitated hematoma removal and ameliorated permeability of blood brain barrier in hemorrhagic rats,which was maybe related to the upregulation of t-PA and downregulation of MMP-9 by the Chinese herbs.

关 键 词：脑出血 纤溶酶原激活物 基质金属蛋白酶9 凉血通瘀方 大鼠 

分 类 号：R285.5[医药卫生—中药学]