簇毛麦易位系V9125-2抗条锈基因YrWV的遗传分析和SSR分子标记  

Genetic Analysis and SSR Molecular Mapping of New Stripe-Rust Resistance Gene YrWV Derived from Triticum aestivum-Haynaldia villosa Translocation Line V9125-2

作　　者：王睿[1] 张书英[1] 徐中青[1] 陈洁[2] 李强[1] 侯璐[1] 井金学[1] 

机构地区：[1]西北农林科技大学植物保护学院/陕西省农业分子生物学重点实验室,陕西杨凌712100 [2]河北省农林科学院植物保护研究所,河北保定071000

出　　处：《中国农业科学》2011年第1期9-19,共11页Scientia Agricultura Sinica

基　　金：教育部高等学校学科创新引智计划(B07049);科技部小麦重大病虫害防控技术(2006BAD08A05);农业部小麦条锈病菌毒性变异与条锈病综合治理研究示范项目(200903035-02)

摘　　要：【目的】对高抗条锈病的簇毛麦易位系V9125-2进行研究,明确其抗病性遗传特点,并对其抗条锈病基因定位,为选育优质抗源材料提供依据。【方法】采用中国当前流行的7个条锈菌生理小种CYR29、CYR30、CYR31、CYR32、CYR33以及Su11-4、Su11-11对簇毛麦易位系V9125-2和铭贤169的杂交后代进行苗期抗条锈性遗传分析。以接种CYR29的F2抗感分离群体为研究对象,应用BSA法用289对普通小麦的SSR标记引物对V9125-2进行SSR分析,并用F3群体验证标记连锁性。用黄淮麦区主栽品种检测与V9125-2抗条锈基因的同源性。【结果】易位系V9125-2对CYR29的抗病性由1对显性基因控制;对CYR30、CYR32、CYR33以及Su11-11的抗病性由一显一隐2对基因控制;对CYR31以及Su11-4的抗病性由2对独立的显性基因控制。从289对SSR引物中筛选到6对与抗病基因YrWV(暂命名)连锁的多态性微卫星标记:Xbarc87、Xwmc463、Xwmc405、Xbarc126、Xwmc438和Xgwm473,其遗传距离分别为9.1、3.9、5.1、12.6、29.0和57.4 cM,位于小麦染色体7DS上。经F3群体验证,6个标记与YrWV连锁。V9125-2抗条锈基因YrWV与检测品种的抗条锈基因同源率极低。【结论】具有6个多态微卫星标记的抗条锈基因YrWV位于小麦7D染色体短臂上,其可能是一个来自簇毛麦的新基因。[Objective] The high stripe rust resistance material, translocation line V9125-2, from Triticum aestivum-Haynaldia villosa was studied. Inheritance, location and SSR molecular mapping of stripe rust resistance genes in V9125-2 are important for providing a basis for breeding high stripe rust resistance materials. [Method] To identify and tag new resistance genes to stripe rust in the translocation line V9125-2 from Triticum aestivum-Haynaldia villosa, the yellow rust races （Puccinia striiform f.sp. stritici） CYR29, CYR30, CYR31, CYR32, CYR33, Sull-4 and Sul 1-11 were selected as innoculum of genetic analysis of resistance to stripe rust in the progeny population of V9125-2xMingxian169 at seedling stage. Using the F2 population inoculated with CYR29 to test SSR analysis, combined with bulked segregation analysis （BSA）, 289 pairs of primers were used. The F3 population were used to test the linked markers. And the homologous rate between the stripe rust resistance genes of V9125-2 and the varieties in Huang-Huai wheat grown region was detected. [Result] The results showed that one dominant gene conferred resistance to stripe rust race CYR29. One dominant gene and one recessive gene indenpently conferred to CYR30, CYR32, CYR33 and Sul 1-11. And two pairs of independent dominant genes conferred to CYR31 and Sul 1-4, respectively. The F2population inoculated with CYR29 were used for SSR analysis, temporarily designated as YrWV. SSR markers, as well as 289 pair primers, combined with bulked segregation analysis （BSA） revealed that six SSR markers, Xbarc87, Xwmc463, Xwmc405, Xbarc126, Xwmc438 and Xgwm473, located on chromosome 7DS were linked to YrWV, and their genetic distances to YrWV were 9.1, 3.9, 5.1, 12.6, 29.0 and 57.4cM, respectively, and the six sites linked to YrWV were validated by a segregated ratio of F3 lines. These markers would be used for breeding new wheat cultivars with marker-assisted selection. A very low homologous rate was found in 43 varieties in Huang-Huai wheat grown region tested w

关 键 词：簇毛麦 普通小麦 易位系 条锈病 遗传分析 SSR标记 

分 类 号：S512.1[农业科学—作物学]