RP-HPLC法测定小鼠血浆中10-羟基喜树碱的浓度  

Determination of the Plasma Concentration of 10-hydroxycamptothecin in Mice by RP-HPLC

作　　者：郭宏红[1] 郑健[1] 井立佳[1] 李思阳[1] 阎秀峰[1] 王洋[1] 

机构地区：[1]东北林业大学盐碱地生物资源环境研究中心,东北油田盐碱植被恢复与重建教育部重点实验室,哈尔滨市150040

出　　处：《中国药房》2011年第1期13-15,共3页China Pharmacy

基　　金：黑龙江省留学归国基金资助项目(LC201004);黑龙江省自然科学基金资助项目(C200605)

摘　　要：目的:建立测定小鼠血浆中10-羟基喜树碱浓度的方法。方法:33只小鼠灌胃给予10-羟基喜树碱80mg·kg-1,于给药前及给药后0.17、0.33、0.67、1、1.5、2、4、8、12、24h时心脏取血,采用高效液相色谱法,测定其中血药浓度。色谱柱为Luna C18,流动相为水-甲醇,梯度洗脱,流速为1.2mL·min-1,内标为喜树碱,荧光检测器检测,激发波长为383nm,发射波长为553nm。结果:内源性杂质不干扰测定,10-羟基喜树碱检测浓度线性范围为1.25～80ng·mL-1(r=0.9997),平均方法回收率为96.46%～101.87%,平均萃取回收率为82.4%～109.6%,日内、日间RSD均≤5.00%。结论:该方法简便可靠、专属性好、精密度高,可用于10-羟基喜树碱的血药浓度检测。OBJECTIVE：To establish the method for the concentration determination of 10-hydroxycamptothecin（HCPT）in mice plasma.METHODS：33 mice were given HCPT 80 mg·kg -1 intragastrically.The blood samples of heart were collected before administration and 0.17 h,0.33 h,0.67 h,1 h,1.5 h,2 h,4 h,8 h,12 h,24 h after administration.The plasma concentration of HCPT was determined on Luna C18 column with mobile phase consisted of water-methanol（gradient elution）at the flow rate of 1.2 mL·min-1 .Fluorescence detector with excitation and emission wavelengths of 383 nm and 553 nm was used for the determination of HCPT.Camptothecin was used as internal standard.RESULTS：Endogenous impurity did not interfere with the determination. The linear range of HCPT were 1.25～80 ng·mL-1 （r=0.999 7）.The average assay recovery was 96.46%～101.87% and extraction recovery was 82.4%～109.6%.The intra-day and inter-day precision were less than 5.00%.CONCLUSION：The method is simple,reliable,specific and sensitive.It is suitable for plasma concentration of HCPT.

关 键 词：高效液相色谱法 小鼠 血浆 10-羟基喜树碱 浓度 

分 类 号：R969[医药卫生—药理学] R979.1[医药卫生—药学]