基于多壁碳纳米管和纳米金修饰电极测定特殊序列DNA的电化学生物传感器的制备  

Preparation of a new sequence-specific DNA electrochemical biosensor based on the multi-walled carbon nanotubes and gold nanoparticles modified electrodes

作　　者：何月珍[1] 汪杰[1] 盛杰[1] 张玉忠[1] 

机构地区：[1]安徽师范大学化学与材料科学学院,芜湖241000

出　　处：《分析试验室》2011年第1期20-22,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20675002)项目资助

摘　　要：基于多壁碳纳米管和纳米金复合膜修饰电极制备了特殊序列的靶DNA的电化学生物传感器。该传感器以六氨基合为杂交指示剂,用差示脉冲伏安法进行检测DNA杂化,其响应信号与靶DNA浓度在1.0×10-12～1.0×10-7mol/L范围内呈线性关系,检测限达3.5×10-13mol/L。该传感器能区分单碱基错配的靶DNA.A new electrochemical biosensor for sequence-specific target DNA detection was prepared with a multi-walled carbon nanotubes and gold nanoparticles composite film modified electrode.Differential pulse voltammetry was used to monitor DNA hybridization by measuring the electrochemical signals of [Ru（NH3）6]3＋ bound to double-stranded DNA.This DNA biosensor can detect the target DNA quantitatively in the range of 1.0 × 10-12-1.0 × 10-7 mol/L,with a detection limit of 3.5 × 10-13 mol/L（S/N=3）.In addition,the DNA biosensor exhibits excellent selectivity,even for single-mismatched DNA detection.

关 键 词：DNA 多壁碳纳米管 金纳米粒子 差示脉冲伏安法 

分 类 号：O657.1[理学—分析化学]