铁氰化钾-Fe(Ⅲ)分光光度法测定盐酸氯丙嗪  

Spectrophotometric determination of chlorpromazine hydrochloride using potassium ferricyanide-Fe(Ⅲ)

作　　者：刘礼涛[1] 李晶[1] 李全民[1] 

机构地区：[1]河南师范大学化学与环境科学学院,新乡453007

出　　处：《分析试验室》2011年第1期41-43,共3页Chinese Journal of Analysis Laboratory

基　　金：河南省科技厅自然科学基金(082300420210)项目资助

摘　　要：建立了以铁氰化钾-Fe(Ⅲ)体系测定盐酸氯丙嗪的新方法。研究表明,盐酸氯丙嗪可以使Fe(Ⅲ)还原为Fe(Ⅱ),还原生成的Fe(Ⅱ)可以与K3[Fe(CN)6]反应生成可溶性普鲁士蓝KFe[Fe(CN)6]。盐酸氯丙嗪的质量浓度在0.21～32.00μg/mL范围内与吸光度呈现良好线性关系,线性回归方程A=0.01854+0.07652ρ(μg/mL),相关系数R=0.9992,摩尔吸光系数ε=2.5×104·L·mol-1·cm-1,检出限0.12μg/mL。方法用于测定药物和血清中盐酸氯丙嗪含量,回收率为98.1%～101.3%。A novel spectrophotometric method has been established to determine chlorpromazine hydrochloride using potassium ferricyanide-Fe（Ⅲ）.It has been demonstrated that Fe（Ⅲ） is reduced to Fe（Ⅱ） by chlorpromazine hydrochloride,and in situ formed Fe（Ⅱ） reacts with potassium ferricyanide to form soluble prussian blue（KFeⅢ[FeⅡ（CN）6]）.Beer＇s law is obeyed in the range of 0.21～32.00 μg/mL for chlorpromazine hydrochloride at the maximal absorption wavelength of 735 nm.The linear regression equation is A=0.01854 ＋ 0.07652ρ（μg/mL） with a correlation coefficient of 0.9992 and the apparent molar absorption coefficient of 2.5 × 104 L/mol cm.The detection limit is 0.12 μg/mL.This method has been successfully applied to the determination of chlorpromazine hydrochloride in pharmaceutical and serum samples with satisfactory results.

关 键 词：Fe(Ⅲ) 铁氰化钾 盐酸氯丙嗪 分光光度法 

分 类 号：O657.3[理学—分析化学]