HPCE法同时测定蒲公英中的四种组分  

Simultaneous determination of four components in dandelion by HPCE

作　　者：刘成伦[1] 丁世环[1] 谢太平[1] 杨琦[1] 吕元琦[2] 

机构地区：[1]重庆大学化学化工学院,重庆400044 [2]德州学院分析测试中心,德州253023

出　　处：《分析试验室》2011年第1期63-65,共3页Chinese Journal of Analysis Laboratory

基　　金：重庆市科委自然科学基金项目(CSTC;2007BB0200);重庆大学"211工程"三期创新人才培养计划项目(S-09103)资助

摘　　要：建立了高效毛细管电泳(HPCE)同时测定蒲公英中黄酮类化合物、咖啡酸和绿原酸的方法。在优化条件下,4种组分在10 m in内得到良好分离。绿原酸,异槲皮甙,槲皮素,咖啡酸的线性浓度范围分别为0.003～0.2,0.003～0.2,0.001～0.1,0.001～0.1mg/mL,线性相关系数分别为0.9999、0.9999、0.9998和0.9994。4种组分基于迁移时间和峰高的相对标准偏差分别为0.19%、0.34%、0.20%、0.23%和1.8%、2.6%、2.8%、2.7%。检出限分别为0.56、0.56、0.20和0.21μg/mL。方法可用于中国传统医学中的药物分析。A method for simultaneous determination of flavonoid,caffeic acidand chlorogenice acid in dandelion by HPCE was proposed.The results show that four components could be well separated in 10 minutes under the optimized conditions.Good linearity of four analytes was found in range of 0.003～0.2,0.003～0.2,0.001～0.1,0.001～0.1 mg/mL with correlation coefficients of 0.9999,0.9999,0.9998 and 0.9994 for chlorogenic acid,isoquercitrin,quercetin,coffeic acid,respectively.The relative standard deviations of migration time and the peak areas of four components were 0.19%,0.34%,0.20%,0.23% and 1.8%,2.6%,2.8%,2.7% and the detection limits were 0.56,0.56,0.20 and 0.21（g/mL） respectively.The method will be successfully applied to the determination of drug in traditional Chinese medicine.

关 键 词：高效毛细管电泳 蒲公英 黄酮类化合物 咖啡酸 绿原酸 

分 类 号：O657.8[理学—分析化学]