高效液相色谱-串联质谱法检测中药材中有机磷农药残留量  

Determination of multi-residues of organophosphorus pesticides in chinese medicinal herbs by HPLC-MS/MS

作　　者：王海涛[1] 张睿[1] 姚燕林[1] 王维志[1] 刘庆斌[2] 段宏安[1] 王沫[2] 郑小燕 

机构地区：[1]连云港出入境检验检疫局,连云港222042 [2]华中农业大学,武汉430070 [3]湖北省食品药品监督检验研究院,武汉430064

出　　处：《分析试验室》2011年第1期72-75,共4页Chinese Journal of Analysis Laboratory

基　　金：江苏出入境检验检疫局科技项目(2009KJ30)资助

摘　　要：研究了中药材中有机磷农药残留量的高效液相色谱-串联质谱同步检测方法。采用CAPCELL PAK MG C18反相柱,以乙腈为提取溶液,以Carb/PSA柱为净化柱,液相色谱-串联质谱仪测定。方法线性范围为10～500μg/L,11种有机磷农药在此范围内线性良好,相关系数为0.9961～0.9999。在10～100μg/kg浓度范围内,加标回收率在70%～110%之间,相对标准偏差为1.4%～11%,最低检出限为2～20μg/kg,符合残留检测分析要求。A rapid analytical method based on high performance liquid chromatography tandem mass spectrometry（HPLC-MS/MS） for detection of organophosphorus pesticides in five Chinese medicinal herb（Gardenia jasminoides Ellis、Plantago asiatica Linn、Radix Scrophulariae、Gly-cyrrhiza uralensis Fisch and Linicera japonica） was developed.Organophosphorus pesticides were extracted by acetonitrile from the Chinese medicinal herb samples and cleaned up with Carb/PSA cartridge,then were detected by HPLC-MS/MS.The linear ranges of 11 organophosphorus pesticides were 10-500 μg/L.In the range of 10-100 μg/kg,the average recoveries of 11 organophosphorus pesticides achieved 70%,the limits of detection were 2～20 μg/kg.It was in accordance with the requirements for simultaneous determination of 11 organphosphorus pesticides.

关 键 词：高效液相色谱-串联质谱 中药材 有机磷农药 多农药残留 

分 类 号：O657.63[理学—分析化学]