高效液相色谱法测定人血浆中多潘立酮的浓度  

Determination of Domperidone in Human Plasma by HPLC

作　　者：纪宏宇[1,2] 曲福军[1,2] 刘红梅[1,2] 李湘晖[1,2] 黄丽军[1,2] 

机构地区：[1]哈尔滨医科大学附属第二医院药学部,哈尔滨150086 [2]黑龙江省高校重点实验室

出　　处：《中国药师》2011年第1期80-81,共2页China Pharmacist

摘　　要：目的:建立测定人血浆中多潘立酮浓度的高效液相色谱法。方法:以普萘洛尔为内标,取血浆样品用0.1 mol·L^(-1)氢氧化钠溶液碱化后乙醚提取,再用0.2 mol·L^(-1)盐酸溶液提取乙醚中药物,取20μl进行HPLC测定。色谱柱:Diamonsil C_(18)(200 mm×4.6 mm,5μm),流动相:乙腈-0.02 mol·L^(-1)磷酸盐缓冲液(pH 3.5)(25:75),荧光检测器激发波长:282nm;发射波长:326nm,流速:1.0 ml·min^(-1),柱温:25℃。结果:标准曲线的线性范围为1.0～100.0μg·L^(-1)(r=0.999 2),相对回收率在99.1%～108.8%之间,日内和日间RSD均小于10%。结论:该方法具有操作简便可靠,准确,稳定性高等特点,适用于多潘立酮血药浓度的测定。Objective ： To develop an HPLC method for determination of domperidone in human plasma. Method： Propranolol was used as the internal standard. After alkalified by 0. 1 mol. L-1 NaOH, plasma was extracted by diethyl ether, then drug in diethyl ether was extracted by 0. 2 mol.L-1 HC1 and determined by HPLC. A Diamonsil C18 column （200 mm ×4. 6 mm,5μm） was used. Mobile phase was a mixture of acetonitrile and 0. 02 mol. L^- 1 KH2 po4 buffer （ pH 3.5 ） （25 ： 75 ）. The excitation wavelength and emission wavelength was 282 nm and 362 nm,respectively. The flow rate was 1.0 ml.min^-1 and the column temperature was 25 ~C. Result： Calibration curves were linear within the concentration range of 1.0-100. 0 μg·L^-1. The recovery was 99. 1%-108. 8%. The intra-day and inter-day RSD were both less than 10%. Conclusion： This method is simple, accurate, stable and suitable for determination of domperidone in human plasma.

关 键 词：多潘立酮 高效液相色谱法 血药浓度 

分 类 号：R969.1[医药卫生—药理学]