诱导子对拟茎点霉139产去乙酰真菌环氧乙酯的调节作用  

Effect of Elicitors on Deacetylmycoepoxydiene Metabolite Produced by Phomopsis sp.139

作　　者：袁丽峰[1] 黄耀坚[1] 郑忠辉[1] 沈月毛[1] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2011年第1期101-105,共5页Journal of Xiamen University：Natural Science

基　　金：国家海洋863项目(2007AA091503)

摘　　要：去乙酰真菌环氧乙酯(DAM)是红树植物秋茄内生真菌拟茎点霉A123菌株产生的次级代谢产物,为了进一步提高DAM的产量,选择和制备了13种诱导子,对具有较强抗肿瘤活性的139菌株进行诱导,考察诱导子对菌株产DAM的调节作用.结果表明,白色假丝酵母菌(Candida albicans)细胞壁制备物和壳聚糖具有显著的正调节作用;采用CCD设计响应面实验获得最佳诱导条件为:发酵144 h后加入100 mg/L壳聚糖,通过高效液色谱仪测定DAM最高产量,其峰面积可达2 296.38,比同批空白对照提高36%.Deacetylmycoepoxydiene（DAM） is a kind of second metabolic products of the endophytic fungul A123,containing an oxygen-bridged cyclooctadiene.DAM has high anti-tumor activity.In order to improve the production of DAM,thirteen elicitors were used to treat Phomopsis sp.139,a mutant strain of A123.The results showed that the Candida albicans cell wall material and chitosan had a significant influence on DAM production.Then through response surface experiments,the optimal elicition conditions were obtained.It was that chitosan（100 mg/L） was added after 144 h cultivation.The maximum yield was expected to reach 2 296.38,which increased 36% compared to the blank.

关 键 词：诱导子 去乙酰真菌环氧乙酯 壳聚糖 

分 类 号：Q93[生物学—微生物学]