高效好氧反硝化菌的紫外诱变育种  

Mutation Breeding of Aerobic Denitrifiers by UV-irradiation

作　　者：钟君伟[1,2] 鄢庆枇[1,2] 纪荣兴[1,2] 朱苏琴[1,2] 

机构地区：[1]集美大学水产学院,福建厦门361021 [2]福建省高校水产科学技术与食品安全重点实验室,福建厦门361021

出　　处：《集美大学学报（自然科学版）》2011年第1期14-19,共6页Journal of Jimei University：Natural Science

基　　金：国家863计划项目(2007AA09Z115);厦门市科技项目(3502Z20073019;3502Z20073020)

摘　　要：采用紫外诱变法对好氧反硝化菌A762进行诱变处理,根据显色圈大小(G)与菌落直径(C)之比,初筛得到8株突变菌,再根据脱氮效果,从中复筛出1株总氮(TN)去除率最高的突变株B25,并对其好氧反硝化性能进行了研究.结果显示:紫外诱变96 h后,在好氧条件下,相对于原菌株A762,菌株B25具有更好的生长优势,对NO3--N去除率达到90%以上,远高于原菌的22.201%;能短时间内去除积累的亚硝态氮,TN去除率提高到84.627%,比原菌株A762提高了60.071%.在反硝化过程中,培养液pH值逐渐上升,而氧化还原电位(ORP)逐渐降低.虽然诱变株B25有较强的反硝化活性,但还要进一步研究其在养殖水体中的脱氮效果,以便能得到实际应用.An aerobic denitrifier （strain A762） was mutagenized by UV-irradiation and 8 mutants were selected for their higher ratios of G/C. A mutant （B25） with highest rate of TN removal was obtained. The aerobic denitrification characteristics of strains B25 and A762 were investigated. The results showed： compared with strain A762, strain B25 grew fast and had a higher reduction rate of NO3- - N and NO2- - N; dur- ing the denitrification process of both strains, the pH values in the culture medium gradually increased, while the Oxidation-reduction Potential gradually decreased. Strain B25 showed its ability in aerobic denitrification, but more studies should be carried out before its application to aquaculture water.

关 键 词：好氧反硝化菌 紫外诱变 脱氮 

分 类 号：Q93[生物学—微生物学]