贝氏隐孢子虫鸡胚和雏鸡增殖模型筛选抗隐孢子虫药物试验  

Evaluation of curative anticryptosporidial activity of four kinds of potential drugs in chick embryo and chicken

作　　者：张素梅[1] 赵金凤[1] 黄磊[1] 张龙现[1] 宁长申[1] 菅复春[1] 齐萌[1] 王俊霞[1] 李寒松[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《中国兽医学报》2011年第1期59-64,共6页Chinese Journal of Veterinary Science

基　　金：国家重大传染病专项"重要寄生虫病检测和监测技术研究"(2008ZX10004-011);国家自然科学基金资助项目(30371863)

摘　　要：为探讨复方磺胺对甲氧嘧啶、磺胺二甲基嘧啶、地克珠利和阿奇霉素4种药物对鸡胚和雏鸡人工感染模型中鸡源贝氏隐孢子虫的作用,筛选有效防治隐孢子虫药物,采用鸡胚培养和人工感染雏鸡模型,分别以各组鸡胚发育状况、鸡胚中卵囊量、雏鸡精神状态、排卵囊规律、体质量变化、发病率、死亡率等为观察指标,考察这4种药物对隐孢子虫的作用。结果显示,使用复方磺胺对甲氧嘧啶、磺胺二甲基嘧啶、地克珠利、阿奇霉素组鸡胚中虫体数分别为阳性对照组的15.05%、88.71%、77.42%、68.82%。复方磺胺对甲氧嘧啶可缓解人工感染雏鸡隐孢子虫病临床症状,感染雏鸡发病率降低20%,死亡率降低13.13%,且可使排卵囊强度降低。结果表明,复方磺胺对甲氧嘧啶以一定剂量连续使用一定时间对雏鸡贝氏隐孢子虫病有潜在治疗作用。To explore the effect of oocyst multiplication and pathogenicity of Cryptosporidium baileyi derived from chicken in chick embryo and chicken by compound sulfamethoxydiazine,sulfadimidine,diclazuril and azithromycin,the anticryptosporidial activity of these potential drugs were observed in chick embryo and chicken infected artificially with C.baileyi oocyst from chicken.The develepment of chick embryo and Cryptosporidium baileyi were both evaluated,and the number of oocysts in chick embryo were counted.In addition,main clinical symptoms of cough,sneezing,dyspnea,pattern of shedding oocysts,weight gains,morbidity and mortality in chicken infected with the oocysts were observed.The results showed that the number of oocysts in chick embryo treated with compound sulfamethoxydiazine,sulfadimidine,diclazuril and azithromycin in comparison to control were 15.05%,88.71%,77.42% and 68.82%,respectively.Moreover,compound sulfamethoxydiazine can reduce morbidity（20%）,mortality（13.13%） and clinical signs of respiratory tract disease observed in chickens orally inoculated 40×104oocysts of Cryptosporidium baileyi at 4 days of age（30 chickens/group）.It was also found that the intensity of oocyst discharge from chickens in treated group with compound sulfamethoxydiazine was lower than that in untreated group.Sulfamethoxydiazine may be recommended in the prevention of avian cryptosporidiosis,as the compound is administered continuously in an adapted dose.

关 键 词：复方磺胺对甲氧嘧啶钠 磺胺二甲基嘧啶钠 地克珠利 阿奇霉素 贝隐孢子虫 

分 类 号：S852.723[农业科学—基础兽医学] R535[农业科学—兽医学]