邻苯二甲酸二甲酯(DMP)在乌鳢体内的代谢动力学和残留  

Metabolic kinetics and residues of Dimethyl phthalate(DMP) in shakehead fish

作　　者：戴贤君[1] 葛建[1] 李明揆[1] 

机构地区：[1]中国计量学院生命科学学院,浙江杭州310018

出　　处：《中国兽医学报》2011年第1期77-80,共4页Chinese Journal of Veterinary Science

基　　金：浙江省自然科学基金资助项目(Y3080255)

摘　　要：采用反相高效液相色谱-紫外检测法测定邻苯二甲酸二甲酯(DMP)在乌鳢体内的代谢动力学和残留。将DMP以20mg/kg灌服乌鳢后,取样测定不同时间点血浆和肌肉中的DMP质量浓度,并利用3p97药物动力学软件对试验数据进行分析,结果表明乌鳢灌服DMP后体内吸收较快,吸收半衰期为0.028h,分布半衰期为1.16h,消除半衰期为231.29h,血浆质量浓度-时间曲线下面积AUC为65.83μg.h.mL-1,达峰时间为0.17h,达峰质量浓度为0.71mg/L;同时在8、12、24、48、72、144h各点肌肉中DMP浓度均比血浆中高,DMP在肌肉组织中的消除半衰期为256.6h,通过代谢动力学和残留研究显示DMP在乌鳢体内吸收和分布较快,消除缓慢。The Dimethyl phthalate（DMP） concentrations in plasma and muscle tissues were determined by the reversed-phase high performance liquid chromatography（RP-HPLC） after a single oral administration at 20 mg/kg for shakehead fish,Ophiocephalus argus Cantor.The metabolic kinetics and residues of DMP were studied with the software 3p97.The results showed that DMP was absorbed very quickly and eliminated very slowly in shakehead fish.The T1/2Ka,T1/2α and T1/2β were 0.028,1.16 and 231.29 h,respectively.The Tpeak and Cmax were 0.17 h and 0.71 mg/L.The elimination half-life of DMP in muscle was 256.6 h,and the concentrations of DMP in muscle were higher than in plasma at the time of 8,12,24,48,72 and 144 h.

关 键 词：乌鳢 邻苯二甲酸二甲酯 反相高效液相色谱 代谢动力学 残留 

分 类 号：S859.7[农业科学—临床兽医学]