慢性综合应激对大鼠肠道Cajal间质细胞的影响  

Effects of chronic and comprehensive stress on interstitial cells of Cajal in adult rats

作　　者：周婷[1] 陈明锴[1] 张丽[1] 沈世强[1] 罗和生[1] 

机构地区：[1]武汉大学人民医院胃肠疾病研究所,430060

出　　处：《中华实验外科杂志》2011年第1期42-44,共3页Chinese Journal of Experimental Surgery

基　　金：湖北省自然科学基金资助项目（2009CDB283）;武汉大学优秀青年教师基金资助项目（3082018）

摘　　要：目的观察慢性综合应激对大鼠行为及Cajal间质细胞（ICC）的影响。方法将雄性Wistar大鼠30只随机分为对照组（15只）和模型组（15只）。模型组接受21d不同的应激，对照组不予任何应激。造模前后行旷场试验。整个实验期间，定期观察大鼠体质量及行为变化。采用透射电镜和免疫荧光法观察各组大鼠ICC超微结构及网络变化。结果模型组大鼠体质量（230．50±14．89）g明显低于对照组（292．50±20．55）g（P〈0．05）；旷场试验显示，模型组大鼠总行程（1407．68±586．92）em和直立次数（7．13±2．50）较正常对照组（6224．58±1918．25）cm、（17．07±5．42）明显减少，差异有统计学意义（P〈0．05）。慢性综合应激使大鼠ICC超微结构发生改变、数量减少、与周围其他ICC、平滑肌和神经末梢之间的缝隙连接显著减少。结论大鼠ICC形态结构改变，可能是慢性综合应激所致功能性胃肠病而引起胃肠运动异常的重要环节。Objective To investigate the effect of chronic and comprehensive stress on the behavior and interstitial cells of Cajal （ICC） of adult rats. Methods Thirty male Wistar rats were randomly di- vided into 2 groups： normal control group （n = 15） and model group （n = 15）. Except the normal group, the model group was given various kinds of stimulation for continuous 21 days. Open-field test was used before and after the chronic and comprehensive stress. During the whole experiment, the body weight and behavior of each rat were observed in periodic days. The ultrastructure and network of ICC in adult rats were observed by using the transmission electron microscopy and immunofluorescenee. Results Compared with the control group, the body weight in the model group was remarkably reduced [ （ 230. 50 ± 14. 89 ） vs （292. 50 ± 20. 55 ） g, P 〈 0. 05 ） ]. Before and after chronic stress, there were significant differences in total ambulation distance and total number of rearings in open-field tests [ （6224. 58 ± 1918. 25 ） vs （ 1407. 68 ± 586.92） cm, （ 17. 07 ± 5.42） vs （7. 13 ± 2. 50）, P 〈 0. 05 ） ]. The ultrastructure of ICC was changed, and the quantity of ICC and gap junctions between ICC and surrounding cells, such as other ICC, smoth muscle cells, and terminal nerves were decreased significantly in the model group as compared with the contro group. Conclusion The changes of ICC may play an important role in abnormal gastrointestinal motility of functional gastrointestinal diseases caused by chronic and comprehensive stress .

关 键 词：功能性胃肠病 CAJAL间质细胞 应激 

分 类 号：R285.5[医药卫生—中药学]