脓毒症易感性与IRAK—M基因多态性的相关性  

Correlation between the susceptivity of sepsis and the polymorphism of IRAK-M

作　　者：罗玉政[1] 宋林学[1] 龚建平[2] 

机构地区：[1]重庆市第九人民医院普外科,400700 [2]重庆医科大学附属第二医院肝胆外科

出　　处：《中华实验外科杂志》2011年第1期51-53,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30500473）

摘　　要：目的探讨脓毒症易感性与IRAK—M基因多态性的关系。方法选择脓毒症患者82例为实验组，118例健康人群为对照组。应用聚合酶链反应（PCR）-限制性片段长度多态性分析法（RFLP）分析IRAK—M＋22148G〉A基因多态性。结果脓毒症组IRAK—M＋22148G〉A位点G／G基因型频率高于对照组（81．7％比28．8％），差异有统计学意义（P〈0．01），G等位基因频率高于对照组（84．1％比33．9％，P〈0．01），差异有统计学意义，脓毒症组肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6水平高于对照组[（843．00±97．34）ng／L比（287．004-79．12）ng／L；（741．00±65．61）ng／L比（194．00±58．47）ng／L]，差异有统计学意义（P〈0．05）；G／G基因型与脓毒症之间有明显相关（OR=11．03，95％CI：5．55～21．94）。结论IRAK—M＋22148G／A基因多态性与脓毒症的易感性相关，G／G基因型者易患脓毒症。Objective To determine the association between the genetic polymorphisms of IRAK-M and the susceptivity of sepsis. Methods Two candidate gene loci in ＋ 22148G 〉 A patients with 82 sepsis infection and 118 heahhy controls were investigated. The polymorphisms were assessed by the polymerase chain reaction （PCR） and the restrict fragment length polymorphisms （RFLP）. Results In sepsis group and control group, the frequency of G/G gene was 81.7% and 8.8% （P 〈0. 01 ） and that of G allele was 84. 1% and 33.9% （P 〈0. 01 ） , respectively. The levels of tumor necrosis factor （TNF） -α and interleukin （IL）-6 in sepsis group were higher than in control group [ （843.00±97.34） vs （287.00 ±79. 12） ng/L; （741.00 ± 65.61 ） vs （ 194. 00± 58.47） ng/L,P 〈 0. 05 ]. The G/G genotype was associated with sepsis （ OR = 11.03,95% CI = 5.55-21.94）. Conclusion The genetic polymorphism of ＋ 22148 site of IRAK-M gene is associated with the susceptivity of sepsis. The G/G genotype is susceptive to sepsis.

关 键 词：脓毒症 基因多态性 

分 类 号：R459.7[医药卫生—急诊医学]