建立一种快速分离人汗腺细胞的实验方法  

Establishment of a quick and facilitate approach to harvest sweat gland cells in vitro

作　　者：许永安[1] 黄沙[1] 付小兵[1] 张翠萍[1] 孙同柱[1] 

机构地区：[1]解放军总医院第一附属医院全军创伤修复重点实验室,北京100048

出　　处：《中华实验外科杂志》2011年第1期54-56,共3页Chinese Journal of Experimental Surgery

基　　金：国家重点基础研究发展计划资助项目（2005CB522603）;国家自然科学基金重点资助项目（30672176、30730090）

摘　　要：目的通过与传统汗腺分离方法进行对比，建立一种新的快速分离、获取人汗腺方法。方法采用制作微粒皮（直径0．5～1．0mm^3）、提高胶原酶Ⅱ型的浓度（2．5g／L）以及改变温度条件（37．5℃）来与传统方法比较，对比两种方法获取汗腺的时间、细胞的形态及增殖情况。结果由此方法分离、获取汗腺细胞所需要的时间明显缩短（由12h以上缩短为3～5h），其形态及增殖特性无明显差异（P〉0．05）。结论一种更加便捷、高效的汗腺细胞分离方法的建立为汗腺的修复与再生研究提供一种快速、大量获取汗腺细胞的新策略。Objective To establish a rapid approach of isolating sweat gland cells （SGCs） compared with the traditional method in vitro. Methods Through diminishing particles of the skin （ diameter was reduced to 0. 5-1.0 mm） , elevating the saturation of the collagenase type Ⅱ（2. 5 g/L） , enhancing the environmental temperature （37.5℃）, the time of harvesting SGCs, the morphology, outgrowth ability, and the proliferation of SGCs obtained by this method were compared with the previous method of obtaining SGCs. Results Compared to previous method, the time of harvesting SGCs by this approach was short- ened obviously from more than 12 h to 3-5 h, meanwhile there was no significant difference in morphology and proliferation ability between them （P 〉 0. 05 ）. Conclusion A rapid and promising method for isolating SGCs in vitrowas established, which supply a new approach of harvesting cells for the study of repair and regeneration of sweat glands.

关 键 词：汗腺细胞 分离 培养 皮肤再生 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]