三七总皂苷对移植静脉再狭窄的影响  

Effects of panax notoginseng saponin on restenosis of vein graft

作　　者：胡敏[1] 张凯伦[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院心脏外科,武汉430022

出　　处：《中华实验外科杂志》2011年第1期63-64,共2页Chinese Journal of Experimental Surgery

摘　　要：目的观察三七总皂苷（PNS）对大鼠移植静脉再狭窄的影响。方法建立SD大鼠颈外静脉颈总动脉旁路移植模型，随机分为假手术组、模型组和PNS组，造模后第2天开始灌胃给药，PNS组给予PNS（100mg／kg），假手术组、模型组灌胃等量蒸馏水（10ml／kg）。给药14d后，取静脉血管桥固定，观察血管内膜增生，免疫组织化学测定增殖细胞核抗原（PCNA）表达及TUNEL法检测平滑肌细胞凋亡。结果上述3组的内膜厚度分别为（4．05±0．85）、（26．30±1．15）、（10．80±0．98）μm；内膜／中膜分别为（0．22±0．09）、（0．76±0．27）、（0．45±0．23），PNS组新内膜厚度明显低于模型组（P〈0．05）；模型组的PCNA表达量（A值）为（23．6±4．3），明显高于PNS组（11．5±3．6，P〈0．05）；模型组的凋亡细胞百分数为（4．3±1．9），明显低于PNS组（20．3±3．5，P〈0．05）。结论PNS可以抑制静脉移植血管桥新生内膜的增生，对预防移植血管再狭窄有一定作用。Objective To observate the effects of panax notoginseng saponin （PNS） on restenosis of rat vein graft. Methods Jugular vein-to-artery bypass grafting was performed on 30 Sprague-Dawley rats. The rats were divided into three groups： sham gruup, model group and PNS group. Drugs were administered at the second day after the operation for 14 days. The grafts were harvested for histoehemieal staining to observe the hyperplasia of neointima, and proliferating cell nuclear antigen （PCNA） expression, and apoptosis of smooth muscle cells were detected by TUNEL. Results In sham group, model group and PNS group, the endometrial thickness was （4. 05± 0. 85 ）, （26. 30 ±1.15 ）, （ 10. 80 ± 0. 98 ） p.m; the intima/media was （ 0. 22 ± 0. 09 ）, （ 0. 76 ± 0. 27 ）, （ 0. 45±0. 23 ）, respectively. The neointimal thickness in PNS group was significantly less than in model group （ P 〈 0. 05 ）. The PCNA expression in model group （A value） was （23.6± 4. 3 ）, significantly higher than in PNS group （ 11.5±3.6 ） （ P 〈 0. 05 ）. The percentage of apoptotic cells in model group was （4. 3 ± 1.9 ） , significantly lower than in PNS group （20. 3 ± 3.5 ） （ P 〈 0. 05 ）. Conclusion PNS can inhibit the neointimal hyperplais of vein graft, which can prevente restenosis after bypass grafts.

关 键 词：静脉移植 三七总皂苷 血管再狭窄 新生内膜 

分 类 号：R285.5[医药卫生—中药学]