依布西林对大鼠无心跳供体肺的保护作用  

Effect of inhaled nitric oxide on the ischemia-reperfusion injury in rat lungs from non-heart-beating donors

作　　者：廖东山[1] 陈昆[1] 余毅[1] 黄庆[1] 李增棋[1] 洪国粦[2] 

机构地区：[1]福建医科大学附属第一医院心外科,福州350005 [2]福建医科大学附属第一医院检验科,福州350005

出　　处：《中华实验外科杂志》2011年第1期105-107,共3页Chinese Journal of Experimental Surgery

基　　金：福建省科技厅青年人才项目（2008F3040）

摘　　要：目的观察依布西林在大鼠尢心跳供体（NHBD）肺保护中的作用。方法将60只SD大白鼠随机分为A组：有心跳供体（HBD）组；B组：NHBD组；C组：NHBD＋依布硒林（Ebselen）组。B组、C组供体处死后维持辅助呼吸，放置室温中30min，再灌注低钾右旋糖苷（LPD）液。受体鼠行“原位左肺移植术”。C组受体在肺移植前1h给予Ebselen。结果C组移植后肺顺应性为0．1740±0．0100，结扎右肺门后15、30min动脉血氧分压分别为（93．97±5．94）、（92．30±6．57）mmHg，肺组织丙二醛（MDA）含量为（0．63±0．23）nmol/mg蛋白，肺组织能量代谢物总量为（821．51±29．70）mol／g，与B组比较差异均有统计学意义（P〈0．05）。结论给予受体一定浓度的Ebselen可改善NHBD肺保护作用。Objective To evaluate the protective effect of ebselen on the rat lungs from non-heartbeating donors （NHBD）. Methods Sixty Sprague-Dawley rats were randomly divided into 3 groups： group A, heart-beating donor; group B, NHBD with 30 rain of warm ischemia time （WIT） ; group C, NHBD with 30 min of WIT and administration of ebselen. The donor lungs in groups B and C maintained ventilation at room temperature for 30 rain after asystolia and then were flushed with LPD solution. The recipient rats underwent left lung transplantation. The recipients in group C were administered with ebselen 1 h before transplantation. Results All the recipients survived during the observation period. The pulmonary compliance of group C was 0. 1740 ±0. 0100. The PaO2 at 15min and 30 rain after the ligation of the right pulmonary hila was （93.97 ± 5.94）, （92. 30 ± 6. 57 ） mmHg, respectively. Malondialdebyde （MDA） of the pulmonary tissue was （0. 63 ± 0. 23 ） nmol/mg pro and the energy metabolism was （821.51 ± 29. 70） mol/g. The difference between group B and group C was significant （ P 〈 0. 05 ）. Conclusion The administration of ebselen is a safe and effective treatment in the preservation of the rat lungs from NHBD.

关 键 词：依布硒林 无心跳供体 肺移植 肺保护 

分 类 号：R655.3[医药卫生—外科学]