雷帕霉素抑制人前列腺癌细胞增殖及其作用机制  

Rapamycin inhibited the proliferation of prostate carcinoma cell line PC-3M-2B4 in vitro

作　　者：郑航[1] 胡伟[1] 郑新民[1] 李世文[1] 王行环[1] 

机构地区：[1]武汉大学中南医院泌尿外科,430071

出　　处：《中华实验外科杂志》2011年第1期124-126,共3页Chinese Journal of Experimental Surgery

基　　金：湖北省自然科学基金资助项目（2007ABA285）

摘　　要：目的观察雷帕霉素（Rapamycin）对体外培养的人前列腺癌PC-3M-2134细胞增殖及凋亡的影响，探讨其机制。方法分别用不同浓度的雷帕霉素（100、200、400、800μg/L）对细胞进行干预后，采用噻唑蓝（MTT）比色法检测细胞增殖变化，流式细胞术检测细胞凋亡变化，Westernblot法检测凋亡相关蛋白bcl-2及hax表达的变化。结果雷帕霉素能明显抑制PC-3M-21M细胞的增殖活性，此作用呈现量-效、时-效关系。雷帕霉素呈浓度依赖性诱导细胞凋亡。雷帕霉素作用PC-3M-21t4细胞后，细胞内凋亡抑制蛋白bcl-2的表达明显降低，bax蛋白的表达明显增加。结论雷帕霉素能够通过调节凋亡相关蛋白bcl-2和bax的表达比例，诱导前列腺癌细胞凋亡，从而抑制肿瘤生长。Objective To investigate the effects of Rapamycin on the growth and apoptosis of human prostate carcinoma cell line PC-SM-21M. Methods The inhibitory effect of Rapamycin was observed at 100,200,400,800μg/L on the growth of human prostate carcinoma cell line PC-3M-2B4 in serum-free medium for different concentrations by methyl thiazol tetrazolium （M3W） assays. Flow cytometry （FCM） analysis was used to study the changes of cell apoptosis. The expression level of bcl-2 and bax was determined by Western blotting. Results Rapamycin caused dose-dependent inhibition on the growth of human prostate carcinoma cell line PC-3M-2B4 in a concentration-and time dependent manner. Rapamycin induced the apoptosis of PC-SM-2B4 cells in a concentration-dependent manner. The levels of bcl-2 protein were reduced gradually with the increase of concentration or action time. Conclusion Rapamycin, a mTOR inhibitor, inhibits the growth of human prostate cancer cell and induces apoptosis of human prostate cancer cell. mTOR might be a potential target for anti-prostate cancer.

关 键 词：哺乳动物雷帕霉素靶蛋白 雷帕霉素 BCL-2 凋亡 信号通路 

分 类 号：R965[医药卫生—药理学]