辐射诱发胸腺淋巴瘤c-mye基因启动子甲基化检测  

Detection on promoter methylation of c-myc gene in thymic lymphomas induced by ionizing radiation

作　　者：于雷[1] 刘永哲[2] 孙世龙[2] 方芳[2] 巩宏伟[2] 陈强[2] 鞠桂芝[2] 

机构地区：[1]吉林大学第二医院放疗科,长春130021 [2]吉林大学公共卫生学院卫生部放射生物学重点实验室

出　　处：《中国公共卫生》2011年第1期81-82,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30670630);吉林大学研究生创新基金资助项目(20091032)

摘　　要：目的研究辐射诱发小鼠胸腺淋巴瘤c-mcy基因启动子CpG岛甲基化状态和mPNA表达的变化。方法 X射线照射BALB/c小鼠建立胸腺淋巴瘤模型,采用硫化测序PCR(bisulfite sequencing PCR,BCP)法和逆转录-聚合酶链反应(RT-PCR)分别检测c-myc基因启动子CpG岛甲基化状态和mRNA的表达。结果辐射诱发胸腺淋巴瘤c-myc基因启动子CpG岛与正常胸腺组织比较呈去甲基化状态,RT-PCR检测结果显示,胸腺淋巴瘤c-myc基因mPNA相对表达量(1.12±0.99)明显高于正常胸腺组织(0.89±0.08),2组比较差异有统计学意义(P<0.01)。结论 c-myc基因启动子去甲基化可能与辐射诱发胸腺淋巴瘤密切有关。Objective To study methylation of CpG islands in promoter region of c-mcy gene and the change of mRNA expression in thymic lymphomas of mice induced by ionizing radiation.Methods The thymic lymphomas model was made with X-ray exposure in BALB/c mice.The methylation of CpG islands in promoter region and the mRNA expression of c-myc gene were detected with bisulfite sequencing PCR and RT-PCR.Results CpG islands in promoter region of c-myc gene were at demethylation state in thymic lymhomas induced by ionizing radiation against normal thymus.The mRNA expression of c-myc gene in thymic lymphomas（1.12±0.99） was higher than that of in normal thymus tissue（0.89±0.08） with a statistical significance（P0.01）.Conclusion There is a correlation between promoter demethylation of c-myc gene and thymic lymphomas induced by ionizing radiation.

关 键 词：胸腺淋巴瘤 甲基化 硫化测序PCR CPG岛 

分 类 号：R763.3[医药卫生—耳鼻咽喉科] Q786[医药卫生—临床医学]