3个紫花苜蓿品种耐盐突变材料的耐盐性评价  

Evaluation on salt tolerance to salt-tolerant variant materials of three alfalfa varieties

作　　者：王珺[1] 柳小妮[1] 

机构地区：[1]甘肃农业大学草业学院草业生态系统教育部重点实验室中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草业科学》2011年第1期79-84,共6页Pratacultural Science

基　　金：国家科技支撑计划"优质高产抗逆苜蓿和饲料作物新品种选育项目(2006BAD04A04-01)"

摘　　要：选取国外耐高温高湿的3个紫花苜蓿(Medicago sativa)品种:"维多利亚"、"盛世"和"CW787",用NaCl作选择剂,采用一步和多步正筛法,筛选出耐盐愈伤组织变异系,对其再生植株的丙二醛、脯氨酸、可溶性蛋白和可溶性糖含量,以及超氧化物歧化酶活性等生理指标进行了测定,并采用隶属函数法对耐盐突变材料的抗盐性进行了综合评价。结果表明,经筛选得到的耐盐突变材料的丙二醛、脯氨酸、可溶性蛋白和可溶性糖含量以及超氧化物歧化酶活性均显著(P<0.05)高于未经筛选的对照植株,其耐盐性大小依次为:"盛世">"CW787">"维多利亚"。Three foreign alfalfa （Medicago sativa） varieties （Victoria, Millennium, and Cw787） with strong resistance to high temperature and humidity were selected to obtain salt-tolerant callus variants by using sodium chloride （NaCl） as selective reagent via positive selection （direct selection and continuous selection） methods. The physiological indexes, including MDA, proline, soluble protein and soluble sugar content, as well as superoxide dismutase （SOD） activity of regeneration plants of callus variants were measured and these physiological indexes was used to comprehensively evaluate the salt tolerance by Subordinate Function method. The results of this study showed that the MDA, proline, soluble protein and soluble sugar content, as well as superoxide dismutase （SOD） activity of regeneration plants of callus variants were significantly （P〈0.05） higher than that of control plants. The order of salt tolerance was Millennium 〉 CW787〉 Victoria.

关 键 词：紫花苜蓿 耐盐突变材料 生理生化指标 耐盐性 模糊隶属法 

分 类 号：S541.9[农业科学—作物学]