灯盏花提取物HPLC指纹图谱研究  

Fingerprint of Herba Erigerontis Extracts by HPLC

作　　者：孙云峰[1] 吴品昌[1] 赵龙山[2] 田宝成[2] 张平 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032 [2]沈阳药科大学药学院,辽宁沈阳110016 [3]北京诺和众生国际生物科技有限公司,北京100036

出　　处：《辽宁中医药大学学报》2011年第1期188-189,共2页Journal of Liaoning University of Traditional Chinese Medicine

摘　　要：目的:用高效液相色谱法建立灯盏花乙醇提取物的指纹图谱分析方法。方法:采用Waters Symmetry ShieldTMR P18色谱柱(5μm,4.6mm×250mm);甲醇-0.4%磷酸水为流动相,线性梯度洗脱,检测波长为335nm。结果:共有22个共有峰。结论:此方法准确、重复性好,为有效地控制灯盏花药材提取物的质量提供了科学的依据。Objective：To establish the HPLC-FPC of Herba Erigerontis extracts.Methods：Based on Waters Symmetry ShieldTMRP18（5μm,4.6mm×250mm）column.,methanol-0.4%phosphate acid was used as the mobile phase,and gradient elution program was applied.The detection wavelength was at 335nm.Results：22 peaks were gained on HPLC fingerprint.Conclusion：This method is simple and accurate with a good reproducibility and can be used as a quality control method for Herba Erigerontis extracts.

关 键 词：灯盏花 提取物 指纹图谱 HPLC 

分 类 号：R122.12[医药卫生—环境卫生学]