HCV抗体阳性样品核酸及核心抗原检测分析  

HCV RNA and Core Antigen(HCV-cAg) Analysis in HCV-Ab Positive Samples

作　　者：龙润乡[1] 杨蓉[1] 白慧珠[1] 李华[1] 王晶晶[1] 廖芸[1] 谭振国[1] 谢忠平[1] 

机构地区：[1]中国医学科学院/北京协和医学院医学生物学研究所,昆明650118

出　　处：《医学研究杂志》2010年第12期41-43,共3页Journal of Medical Research

基　　金：国家"863"资助项目(2007AA02Z480);云南省联合支持国家计划资助项目(2008GA008)

摘　　要：目的对90份临床检测为丙型肝炎病毒抗体(HCV-Ab)阳性样品检测其核心抗原(HCV-cAg)、荧光定量PCR,观察3者间的相关性。方法采集临床诊断为HCV-Ab阳性样品90份,分别用市售的HCV-cAg检测试剂盒、荧光定量PCR检测试剂盒、HCV-Ab检测试剂盒进行3种指标的检测复核。结果 90份临床HCV-Ab阳性样品,检出HCV-cAg阳性25份(27.8%),荧光定量PCR检出67份阳性(74.4%),抗体复核阳性的75份(83.33%);检出率HCV-Ab(83.33%)>荧光定量(74.4%)>HCV-cAg(27.8%)。结论丙肝的3种标志物之间存在一定的联系,但不同试剂的检测结果间存在明显差异。Objective Clinical samples of 90 confirmed HCV - Ab positive cases were detected for core antigen （ HCV - cAg） and HCV RNA by fluorescence quantitative PCR （ FQ - PCR） , and the relativity of the three was observed. Methods 90 samples diagnosed as HCV - Ab positive cases were collected, and the three indicators was detected and rechecked with HCV -cAg ELISA kit, HCV -Ab ELISA Kit and quantitative hepatitis C virus（HCV） PCR Fluorescence Diagnostic kit for sale in market separately. Results Among 90 clinical samples of HCV - Ab positive eases, HCV -cAg was positive in 25 cases （27. 8% ） , FQ - PCR was positive in 67 cases （74.4%） , and antibody rechecking was positive in 75 cases （83.33%）. As regards to the positive rate, HCV -Ab was the highest and florescence quantitative took the second place, and HCV - cAg was the lowest. Conclusion It had some relations among the three mak- ers of HCV, but there were significant differences in detection results of different agents.

关 键 词：丙型肝炎病毒核心抗原 丙型肝炎病毒核酸 丙型肝炎病毒抗体 检测 

分 类 号：R512.62[医药卫生—内科学]