白藜芦醇对兔动脉粥样硬化斑块面积及内膜厚度的影响  

Effects of Resveratrol on the Blood lipid,the Area of Plaque and Thickness of Endomembrane in Rabbits with Atherosclerosis

作　　者：尚秀玲[1] 朱鹏立[2] 蒋娜[1] 徐庆玲[1] 高淑卿[1] 

机构地区：[1]福建省立医院,福州350001 [2]福建省立医院福建省临床老年病研究所

出　　处：《医学研究杂志》2010年第12期49-53,共5页Journal of Medical Research

基　　金：卫生部科学研究基金-福建省卫生教育联合攻关计划项目(WKJ2005-2-017);福建省自然科学基金资助项目(Z0501069)

摘　　要：目的探讨白藜芦醇对AS兔血脂、斑块面积及内膜厚度的影响。方法成熟健康雄性新西兰白兔70只,适应性喂养10天后随机分为5组(按序A、B、C、D、E)。正常对照组给予普通饲料,病理对照组及白藜芦醇干预组给予高脂饲料,干预组同时每天分别给予4mg/kg、8mg/kg、16mg/kg的白藜芦醇。分别在第0周(实验开始前)和第12周末(实验结束时),用全自动生化仪测定血总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平。应用计算机图像分析系统(motic images advanced 3.0图像分析软件)测定斑块面积与内膜面积。结果高脂喂养12周后,病理对照组血脂TC、TG、LDL-C、HDL-C较正常对照组升高(P<0.01);与病理对照组比较,白藜芦醇干预组可降低AS兔血清TG、LDL-C(P<0.01),随剂量的增长降低幅度更大(P<0.01);中、高剂量白藜芦醇干预组HDL-C升高,差异有显著性意义(P<0.01);3种剂量白藜芦醇干预组比较TC无明显变化(P>0.05);高脂喂养12周后,成功建立AS模型,病理对照组有严重的AS病理改变,正常对照组无AS斑块,白藜芦醇干预组AS病变明显减轻,斑块面积明显减少(P<0.05),内膜厚度明显降低(P<0.05),并呈一定的剂量依赖性。结论白藜芦醇可改善脂质代谢紊乱,降低TG和LDL-C水平,升高HDL-C水平,继而抑制血管内膜的增生及斑块的形成,从而发挥抗动脉粥样硬化的作用。Objective To investigate the effect of resveratrol on the blood lipid, area of pathological lesions, thickness of endomembrane in rabbits with atherosclerosis. Methods Seventy male New Zealand white rabbits were randomly divided into 5 groups：A for normal control group, B for pathological control group, C for pathological control group treated with low dosage resveratrol [4mg/（ kg·d） ] , D for pathological group treated with mid dosage resveratrol [ 8rag/（ kg · d） ] , E for pathological group treated with high dosage resveratrol [ 16mg/（kg · d）1. Blood glucose and serum lipids levels were measured on the 0,12th weeks. At the end of the 12th week,aortae was analyzed by histopathological examination. The area of plaque and thickness of endomembrane were determined with Motic images ad- vanced 3.0. Results （ 1 ） The blood lipid levels （ TC, TG, LDL - C and H DL - C） in pathological control group were higher than those in normal control（P 〈0.01 ）. High, mid and low dose of RES could depress the TG and LDL - C of AS rabbits（P 〈0.01 ） ,and the decrea- sing amplitude in high dose of RES group was maximal. High and mid dose of RES could heighten the HDL - C of AS Rabbits（P 〈 0.05）. （2） The atherosclerosis model with rabbits had been successfully established with high fat diet. There were severe pathological lesions in group B. Resveratrol could inhibit the development of atherosclerosis. The pathological changes of atberosclerosis in group C, D and E were significantly milder than those in group B （ P 〈 0.05）. Resveratrol could greatly diminish the area of plaque and thickness of eodomembrane in dose - dependent way. Conclusion Resveratrol could improve dyslipidemia, depress the TG and LI）I, - C of AS rab- bits,and heighten the HDI. - C of AS Rabbits,so it could greatly＊ diminish the area of plaque and thivkness of endomembrane in dose - de- pendent way.

关 键 词：动脉粥样硬化 白藜芦醇 新西兰白兔斑块面积 内膜厚度 

分 类 号：R972.6[医药卫生—药品]