烫伤大鼠组织肿瘤坏死因子-α受体基因表达的变化  

Gene expression of tumor necrosis factor receptor in rats after thermal injury

作　　者：方文慧[1,2] 姚咏明[1,2] 施志国[1,2] 于燕 吴叶[1,2] 黄志红 吴玉萍[1,2] 

机构地区：[1]解放军第三○四医院创伤外科中心 [2]解放军第二○八医院外四科

出　　处：《中国危重病急救医学》1999年第6期361-363,共3页Chinese Critical Care Medicine

基　　金：中国工程院科技奖励基金;全军"九五"医药卫生科研基金

摘　　要：目的：观察烫伤后机体主要脏器肿瘤坏死因子α（ＴＮＦα）及其受体（ＴＮＦＲ）ｍＲＮＡ表达的变化规律及二者的平衡关系。方法：采用大鼠３５％Ⅲ度烫伤模型，分别于伤前、伤后１２、２４、４８、７２小时活杀动物。留取组织和血标本分别检测组织ＴＮＦα、ＴＮＦＲｍＲＮＡ表达及器官功能指标。结果：烫伤后组织ＴＮＦα基因表达明显增强，而ＴＮＦＲｍＲＮＡ表达则呈持续降低趋势。同时，烫伤后各组织ＴＮＦα／ＴＮＦＲｍＲＮＡ比值显著增加，其中以肺组织升高最为显著。相关分析表明，肝组织ＴＮＦＲｍＲＮＡ表达、ＴＮＦα／ＴＮＦＲｍＲＮＡ比值与血清谷草转氨酶（ＧＯＴ）呈显著相关（Ｐ＜０．０５和Ｐ＜０．０１），肾组织ＴＮＦＲｍＲＮＡ表达与血清肌酐（ＳＣｒ）呈显著负相关（Ｐ＜０．０１）。结论：烫伤可明显上调机体主要脏器ＴＮＦαｍＲＮＡ的表达，而ＴＮＦＲ基因表达则呈下调反应，提示ＴＮＦα和ＴＮＦＲ比例严重失调可能参与了烧伤后多器官功能损害的病理生理过程。Objective :To investigate changes in tumor necrosis factor (TNF ) and tumor necrosis factor receptor (TNFR) gene expression in vital organs,and their significance in the pathogenesis of systemic inflammation following burns. Methods :Wistar rats were subjected to a 35% full thickness scald injury,then sacrificed at preburn,and 12,24,48,72 hours postburn,respectively.Tissue samples from liver,kidneys,lungs and intestine were collected to measure TNF and TNFR mRNA expression.In addition,blood samples were obtained for measurement of organ function parameters. Results :Gene expression of TNF elevated markedly after thermal injury,otherwise,TNFR mRNA expression was shown to reduce throughout the observation period.Thus,the ratio of TNF to TNFR mRNA was significantly increased postburn,particularly in pulmonary tissue (67 fold).In addition,it was noted that significant correlations between TNFR or ratio of TNF /TNFR mRNA expression in liver tissue and serum glutamic oxaloacetic transaminase enzyme(GO (P<005 and P<001).Similar results were also obtained between renal TNFR mRNA and serum creatinine levels (P<001). Conclusions :Thermal injury per se can markedly up regulate TNF gene expression and down regulate TNFR mRNA expression in various organs.Imbalance of TNF to TNFR may be involved in the development of multiple organ damage following burns.

关 键 词：烧伤 内毒素 TNF-Α 受体 基因表达 MODS 烫伤 

分 类 号：R644.02[医药卫生—外科学]