银杏查尔酮合成酶基因启动子(GbCHSP)调控元件及功能分析  

Regulatory Elements and Functional Analysis of Chalcone Synthase Gene Promoter from Ginkgo biloba L.

作　　者：李琳玲[1] 程华[1] 程水源[1] 许锋[2] 王燕[2] 姜德志[2] 

机构地区：[1]黄冈师范学院生命科学与工程学院,湖北黄冈438000 [2]长江大学园艺园林学院,湖北荆州434025

出　　处：《园艺学报》2010年第12期1919-1928,共10页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30971974);湖北省自然科学基金重点项目(2008CDA061)

摘　　要：通过染色体步移方法从银杏(Ginkgo biloba L.)基因组中克隆到查尔酮合成酶基因(CHS)翻译起始位点上游1711bp的启动子序列。生物信息学分析表明,该启动子片段中存在多个顺式作用元件,包括紫外/蓝光响应单元、植物激素响应单元、真菌诱导元件、MYB结合位点、TATA-box和CAAT-box等。亚克隆了CHS转录起始位点上游1402bp序列,将其与GUS基因构建融合表达载体pBI121+CHSP,以pBI121-35S作为负对照,通过农杆菌(LBA4404)介导法分别转入烟草。结果表明,银杏CHS启动子序列能驱动GUS基因在烟草中的表达,表达具有组织特异性。GbCHSP的功能研究将有助于揭示银杏叶黄酮的积累与GbCHS基因表达的分子机理。The regulative sequence（1 711 bp）of chalcone synthase gene promoter（CHSP）from Ginkgo biloba L. was cloned by genomic walking. In silico analysis suggested that the sequence contained several typical cis-acting elements,including UV/blue light responsive elements,phytohormone responsive elements,fungal elicitor responsive elements,MYB binding site,TATA-box and CAAT-box. A 1 402 bp promoter sequence upstream 5′ of translation start site of GbCHS was cloned and designated as GbCHSP, respectively. pBI121 ＋ CHSP and pBI121-35S were constructed and transformed into tobaccos by LBA4404. These results showed that pBI121 and pBI121 ＋ CHSP both could drive the transient expression of GUS in tobaccos and pBI121 ＋ CHSP expressed differentially in root,stem and leaf tissues of tobacco. These results will be help to understand the transcriptional regulatory mechanism on GbCHS expression and flavonoids accumulation.

关 键 词：银杏 查尔酮合成酶基因启动子 GUS 调控元件分析 

分 类 号：S664.3[农业科学—果树学]