那贺川野菊的离体保存  

Studies on in Vitro Conservation of Chrysanthemum yoshinaganthum

作　　者：兰伟[1,2] 陈素梅[1] 尹冬梅[1] 陈发棣[1] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]阜阳师范学院生命科学学院,安徽阜阳236041

出　　处：《园艺学报》2010年第12期2007-2016,共10页Acta Horticulturae Sinica

基　　金：教育部新世纪优秀人才支持计划项目(NCET-06-0489);农业部‘948’滚动项目(2008G3);公益性行业(农业)科研专项项目(200903020);安徽省高等学校省级自然科学研究项目(KJ2010B429)

摘　　要：以那贺川野菊为试材,研究了不同浓度蔗糖和多效唑（PP333）对试管苗离体保存的影响,并对保存材料再生后代的遗传稳定性进行了分析。结果表明：常温（23±2）℃、光照强度2000-3000lx、光照时间12h·d-1的培养条件下,在MS＋2.0mg·L-1KT＋0.1mg·L-1NAA＋6.5g·L-1琼脂培养基中,蔗糖浓度为15-30g·L-1时附加6-9mg·L-1PP333能保存试管苗360d以上,存活率达93.53%-100%,且恢复生长后试管苗长势良好,其再生后代的形态特征、过氧化物酶（POD）酶谱和ISSR-PCR扩增图谱与对照相比没有明显差异。The effects of different concentrations of sucrose and PP333 on in vitro conservation of Chrysanthemum yoshinaganthum were investigated,and the genetic stability of the plants regenerated from the in vitro preserved germplasm was tested too. The results showed that plantlets can be conserved in vitro for 360 days in the medium MS ＋ KT 2.0 mg · L-1 ＋ NAA 0.1 mg · L-1 ＋ agar 6.5 g · L-1 supplemented with 15–30 g · L-1 sucrose and 6–9 g · L-1 PP333 under temperature（23 ± 2）℃, illumination intensity 2 000–3 000 lx and 12 h · d-1 photoperiod. The survival rate of plantlets was 93.53%–100%. The plantlets grew well after growth recovery. There were no significant differences in the morphology,peroxidase isoenzyme patterns and ISSR-PCR amplification profiles between the plants regenerated from in vitro preserved plants and non-preserved control,suggesting in vitro conservation didn’t affect the genetic stability of the germplasm.

关 键 词：菊 那贺川野菊 离体保存 遗传稳定性 

分 类 号：S682.11[农业科学—观赏园艺]