浙江地区副猪嗜血杆菌分离株的生物学特性  

Biological characterization of Haemophilus parasuis isolated in Zhejiang area

作　　者：何海健[1] 陆国林 熊永忠[3] 

机构地区：[1]金华职业技术学院农学院,浙江金华321007 [2]浙江地区畜牧兽医局,浙江杭州310029 [3]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2010年第12期941-944,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：浙江地区科技计划项目(2007C12051);浙江地区金华市科技计划项目(2007-2-041)

摘　　要：为明确浙江地区副猪嗜血杆菌(HPS)病的流行特点,提高对该病的免疫防控能力,本研究对HPS分离株进行生物学鉴定。结果表明,HPS在含有2%小牛血清和16μg/mL NAD的TSB培养基中生长良好,在TSB液体培养基中培养16 h～18 h后,活菌数达到峰值;6个分离株对小鼠的LD50分别为2.03×109、5.3×109、1.34×1010、7.0×109、1.17×1010和1.62×109;可在感染HPS豚鼠的心血中分离到该菌;ERIC-PCR指纹图谱分析结果表明,4个分离株与4型、5型和9型标准菌株图谱一致,但有2个分离株与15个血清型标准菌株图谱不吻合。研究提示浙江地区内HPS的散发、流行的原因可能是由于该细菌血清型复杂性造成的。This paper reported the biological characteristics of 6 Haemophilus parasuis field isolates in Zhejiang area.The H.parasuis isolates were cultured in TSB medium containing 2% calf serum and 16 μg/mL NAD,and peak growth was detected after 16 h to 18 h culturing.The median lethal dose（LD50） of these isolates were 2.03×10^9,5.3×10^9,1.34×10^10,7.0×10^9,1.17×10^10,1.62×10^9,respectively.H.parasuis could be isolated from heart blood post experimental infection in guinea pigs.ERIC-PCR fingerprint analysis showed that the genomic fingerprints of these 4 isolates were homologous with 4,5 and 9 serotype,and the other 2 isolates were different from the 15 serovar reference stains.These results indicated that mutiple serotypes of H.parasuis existed in the fields,which could be the cause of H.parasuis outbreaks in Zhejiang area.

关 键 词：副猪嗜血杆菌 生物学特性 动物感染试验 ERIC-PCR 

分 类 号：S852.61[农业科学—基础兽医学]