框镜鲤致病性维氏气单胞菌的分离鉴定  

Isolation and identification of pathogenic Aeromonas veronii from Cyprinus carpio

作　　者：龚倩[1] 高淑琴 单晓枫[1] 郭伟生[1] 孟庆峰[3] 王伟利[3] 钱爱东[1] 

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118 [2]吉林省东辽县卫生学校,吉林东辽136600 [3]吉林出入境检验检疫局,吉林长春130062

出　　处：《中国预防兽医学报》2010年第12期981-983,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：吉林省科技厅项目(20080218);吉林农业大学青年启动基金

摘　　要：为鉴定一株致框镜鲤发病病原菌的生物学特性,本研究由濒死框镜鲤体内分离到一株细菌CY0806,对形态学、培养特性、理化特性等生物学特性以及药物敏感性和16S rRNA序列进行检测和分析。结果显示:细菌株CY0806为革兰氏阴性菌,其理化特性与维氏气单胞菌基本相同。系统进化树分析表明,细菌株CY0806序列与维氏气单胞菌模式株ATCC35624无差异,同源性为100%,因此,鉴定该菌株CY0806为维氏气单胞菌。药物敏感性试验结果显示,庆大霉素、新霉素等11种药物对细菌株CY0806有较好的抑菌效果。A pathogenic bacterial isolate CY0806 was isolated from naturally infected Cyprinus carpio and identified according to morphology,cultural character,physical and chemical characters,16S rRNA sequence analysis methods and drug susceptibility.The results showed that isolate CY0806 was gram negative and the physical and chemical character were identical with A.veronii.Sequence of the bacteria has 100% similarity with that of reference Aeromonas strain ATCC35624.These results demonstrated that isolate CY0806 was A.veronii.Drug susceptibility test results showed that isolate CY0806 was sensitive to gentamicin,neomycin and other 11 drugs.

关 键 词：框镜鲤 维氏气单胞菌 分离鉴定 

分 类 号：S852.61[农业科学—基础兽医学]