脱钙松质骨的骨支架材料制备及其生物学检测  

Preparation and biological detection of demineralized cancellous bone scaffolds

作　　者：杨渊 林春博 陈维平[2] 

机构地区：[1]广西骨伤医院,广西壮族自治区南宁市530012 [2]广西医科大学,广西壮族自冶区南宁市530021

出　　处：《中国组织工程研究与临床康复》2010年第47期8769-8772,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：广西科技厅资助项目(桂科攻0816004-15);广西科技厅资助项目(桂科自0832157),广西科技厅资助项目(桂科攻0592007-1G);广西卫生厅资助项目(重200863)~~

摘　　要：背景：脱钙松质骨的主要成分是胶原,由于其良好的三维空间及促进细胞增殖分化的生长因子,使其具有传导成骨和诱导成骨的双重功能,但是目前没有修订出一个规范科学的制备流程。目的：采用新方法制备兔脱钙松质骨,并检测其孔隙率、降解率及其生物相容性,对其能否作为组织工程的支架材料进行评估。方法：在Urist方法的基础上采用新方法制备新西兰兔脱钙松质骨,并对脱钙松质骨的空隙率、体外降解率、以及细胞活力和诱导骨髓间充质干细胞成骨能力进行检测。实验分为脱钙3h,脱钙24h和脱钙48h组,其他的处理步骤3组都相同。结果与结论：脱钙3,24,48h组的孔隙率分别为76.56%,81.25%,84.38%。完全降解所需时间分别为64,59,53d。细胞在种植后1~3d为生长滞留期,第5天达到对数生长期,以后进入到平台期。诱导后的骨髓间充质干细胞行碱性磷酸酶染色呈阳性。结果提示,新方法制备的脱钙松质骨对细胞无毒害作用,在生物学特性上达到骨组织工程支架材料的要求。BACKGROUND：Collagen is the main component of demineralized cancellous bone,because of its excellent three-dimensional space and growth factors that promote cellular proliferation and differentiation,it has a dual function of bone conduction and bone induction,but there are no amendments of a standard scientific preparation process.OBJECTIVE：To prepare rabbit demineralized cancellous bone,to detect the porosity,degradation rate and biocompatibility,and to investigate the feasibility of demineralized cancellous bone used for tissue engineering scaffolds.METHODS：Based on Urist＇s method,demineralized cancellous bone was prepared using a new method,and the porosity,in vitro degradation rate,cell viability and ossification ability of inducing bone marrow mesenchymal stem cells were determined.The experiment was divided into three groups at decalcification 3,24,48 hours.The other processing steps were the same.RESULTS AND CONCLUSION：The porosity of decalcification 3,24,48 hours was 76.56%,81.25%,84.38%;complete degradation time was 64,59,53 days,respectively.Cells in 1-3 days after incubation were latent phase,entered the logarithmic growth phase at 5 days,then entered the stagnate phase.The induced bone marrow mesenchymal stem cells were stained positive for alkaline phosphatase.The demineralized cancellous bone prepared by new methods has no toxicity and can meet the biological characteristics requirements of bone tissue engineering scaffold materials.

关 键 词：脱钙松质骨 孔隙率 降解率 生物学检测 骨组织工程 

分 类 号：R318[医药卫生—生物医学工程]