改性医用胶为纳米骨胶的体外实验  

In vitro experiment of modified nano-bone adhesive

作　　者：赵喆[1,2] 雷鸣[2] 肖德明[2] 熊建义[2] 马经野[3] 许蕴[3] 陈旭红[3] 彭福宁[3] 

机构地区：[1]广州医学院,广东省广州市510182 [2]深圳市第二人民医院骨科,广东省深圳市518035 [3]深圳市第二人民医院中心实验室,广东省深圳市518035

出　　处：《中国组织工程研究与临床康复》2010年第47期8818-8823,共6页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：广东省科技厅支持项目(2008B030301284)纳米骨胶的实验研究~~

摘　　要：背景：目前α-氰基丙烯酸烷基酯类生物胶可应用于临床许多方面,由于胶本身存在一些缺点,使其在骨科的应用受到限制。目的：对普通医用胶进行改性研究,研制出纳米骨胶并检测其细胞毒性和力学性能。方法：直接在纳米骨胶和普通医用胶上种植兔骨髓基质干细胞细胞,通过倒置显微镜和扫描电镜观察细胞在胶上生长情况。用胶的浸泡液培养细胞,在0,6,12,18,24h时间点和1~7d每天进行细胞活性检测。用两种胶将标准PVC管粘合,比较两种胶的粘合强度,并在不同环境下比较胶凝固时间。结果与结论：经3次换液后细胞可在纳米骨胶和普通医用胶上生长,两种胶对细胞都有一定毒性,但毒性并无明显差别;纳米骨胶的力学强度明显高于普通医用胶,且凝固时间明显快于普通胶。提示经改性后的纳米骨胶细胞毒性不高于普通胶,但力学强度和凝固时间前者明显优于后者,且前者具有促进成骨的作用。BACKGROUND：Currently α-cyanoacrylate glue can be used in many clinical fields,but the application is limited in orthopedics due to some shortcomings of the glue itself.OBJECTIVE：To develop a nano-bone adhesive by modifying common medical glue,to evaluate its cytotoxicity and mechanical property.METHODS：Rabbit bone marrow stromal stem cells were directly implanted with nano-bone adhesive and common medical glue,the growth of these cells on the surface of the two glues was observed by inverted microscope and scanning electron microscope.The extracting solution of the two glues was collected to culture cells.At 0,6,12,18,24 hours（short-term assay,viability）and 1-7 days（long-term assay,survival）,the cells were examined everyday.Standard polyvinyl chloride tubes were cohered by the two glues respectively,to compare their adhesive strength.Their consolidation time on different contract surfaces was also accounted.RESULTS AND CONCLUSION：After three times of culture medium replacement,the cells can be grow on the surface of nano-bone adhesive and medical glue.Both had some cytotoxicity that was not significant difference.The mechanical strength of nano-bone adhesive was stronger than the medical glue,and the consolidation time of the former was also faster than the latter.The cytotoxicity of modified nano-bone adhesive is not higher than the medical glue,but the mechanical strength and consolidation are more superior.The former also can stimulate the ossification.

关 键 词：纳米骨胶 粉碎性骨折 氰基丙烯酸酯 细胞毒性 生物力学 

分 类 号：R318[医药卫生—生物医学工程]