川金丝猴粪样内3种类固醇激素保存时效分析  

Study on Storing Duration of Fresh Feces of Sichuan Snub-nosed Monkey for Extracting Steroids

作　　者：黄英[1] 胡德夫[1,2] 刘树强[1] 张志翔[1,2] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083 [2]国家林业局自然保护区研究中心,北京100083

出　　处：《动物学杂志》2010年第6期64-70,共7页Chinese Journal of Zoology

基　　金：林业公益性项目(No.201004054);国家林业局自然保护区研究中心项目(No.4608101)

摘　　要：川金丝猴(Rhinopithecus roxellanae)是中国特有的灵长类物种,生存于海拔1 400～3 300 m的山地森林地带。采用拾取新鲜粪便的非损伤性途径研究该物种野生种群生理状态,必须首先确定其新鲜粪便在特定保存方法下的保存时效。本项研究探讨在川金丝猴自然分布区夏季可获得的低温(4±1)℃、在95%乙醇中保存条件下,该物种新鲜粪便内3种类固醇激素(睾酮、雌二醇、孕酮)在8个设定天数:0(标准对照)、5、6、7、8、10、20、30 d的保存时效。结果表明,雌性川金丝猴粪样内睾酮和雌二醇在30 d内可稳定保存,孕酮含量在保存10 d时的平均值显著性低于标准参照值(P<0.05);雄性川金丝猴粪样中睾酮含量在保存6 d时的平均值显著低于标准参照值(P<0.05)。本研究说明,在野生川金丝猴新鲜粪便保存条件一致的情况下,该物种新鲜粪样内睾酮、孕酮、雌二醇3种激素保存时效并不一致,实际运用时需要结合研究目的区别对待。Snub-nosed Monkey（Rhinopithecus roxellanae） is an endemic primates in China,which lives in alpine forests from 1 400-3 000 m altitude.Taking their fresh feces as the material of non-invasive method is regarded as a feasible way to study physiologic state of free-ranging population.This work aims to determine the storing duration of feces kept in ethanol for extracting 3 steroids.The fresh fecal samples of snub-nosed monkey were treated with ethanol（95%）,then kept at low temperature（4 ± 1℃） for 0,5,6,7,8,10,20,and 30 d.The fecal testosterone and estradiol of the female were successfully extracted from fecal samples stored within 30 days;while compared with the control samples（stored 0 d）,the content of fecal progesterone of the female and fecal testosterone of the male decreased obviously after having been stored for 10 days and 6 days,respectively.The results showed that,the relative content values of fecal steroid can be used in the comparisons between groups as being preserved in the same condition within 30 days,while the effective storing duration for testosterone,estradiol and progesterone in fresh feces are varied.

关 键 词：非损伤性 粪便 类固醇 保存时效 

分 类 号：Q958[生物学—动物学]