一种新的抗病毒分子HIRRP的相互作用蛋白筛选、验证及生物学功能分析  

A New Anti-viral Molecule HIRRP Protein Interaction Screening,Validation and Biological Function Analysis

作　　者：吴练秋[1] 唐松青[1] 廖芸[1] 张莹[1] 陈婕[1] 李薇[1] 董承红[1] 刘龙丁[1] 李琦涵[1] 

机构地区：[1]北京协和医学院中国医学科学院医学生物学研究所,昆明650118

出　　处：《中国细胞生物学学报》2010年第6期862-867,共6页Chinese Journal of Cell Biology

摘　　要：单纯疱疹病毒1型感染相关抑制蛋白(HSV-1 infection related repress protein,HIRRP)是一个结构和功能仍未知的蛋白。我们前期的工作发现HIRRP能在病毒感染的细胞内高表达,表明HIRRP可能在细胞内参与了抗病毒感染这一重要的生物学事件。然而,HIRRP在细胞内参与抗病毒感染的具体作用机理仍不清楚。为此,我们以HIRRP为诱饵构建了pGBKT7-HIRRP载体,用酵母双杂交技术从人肝细胞cDNA文库中筛选出了玻连蛋白(Vitronectin,VTN),并通过体内免疫共沉淀和双荧光共定位实验,以及体外的促细胞增殖实验和病毒入胞干预实验,证实了HIRRP和VTN之间存在着相互作用。初步的功能分析显示,两者的相互作用,具有协同促进细胞抗病毒的效应。由此,我们得出结论,HIRRP和VTN在细胞内能发生相互作用,并猜测这种作用与细胞的抗病毒感染活动相关。HSV-1 infection related repress protein（HIRRP） is a new protein with unknown structure and function.In our previous work,we found that HIRRP can obtain highly expression in Vero cells infected by HSV-1 and we speculated that HIRRP may take part in the event of anti-infection.However,the underlying mechanisms remain to be fully understood.Vitronectin（VTN） is a candidate molecule hold a relationship with HIRRP by yeast trap.We further demonstrated that VTN can react with HIRRP by Co-IP,fluorescence co-localization,cell proliferation and anti-virus analysis,and this reaction can gain a collaborative effect in anti-infection.Taken together,our data suggest that VTN can make relationships with HIRRP,and this relationship may act a function of anti-infection.

关 键 词：酵母双杂交 单纯疱疹病毒1型感染相关抑制蛋白 玻连蛋白 

分 类 号：R373[医药卫生—病原生物学]