南阳牛Gli3基因多态性及其与生长性状的相关性  

Polymorphisms of Bovine Gli3 Gene and Its Associations with Nanyang Cattle Growth Traits

作　　者：王柯忆[1] 赖新生[1] 张春雷[2] 王鑫磊[1] 于辉[1] 王谋[1] 蓝贤勇[1] 陈宏[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西省农业分子生物学重点实验室,杨凌712100 [2]徐州师范大学细胞与分子生物学研究所,徐州221116

出　　处：《农业生物技术学报》2010年第6期1103-1107,共5页Journal of Agricultural Biotechnology

基　　金：国家高技术研究发展计划(863)项目(No2006AA10Z197,2008AA101010);国家自然科学基金(No30771544,30972080);国家支撑计划(No2008ADB2B03-19);国家转基因重大专项(No2009ZX08009-157B,No2008ZX08007-002);陕西省13115科技创新工程重点专项(No2008ZDKG-11);农业部现代肉牛产业技术体系专项经费共同资助

摘　　要：Gli3基因是Gli-Kruppel家族中的一员,其参与的Sonichedgehog信号通路在动物生长发育,特别是早期生长发育过程中有重要作用。利用PCR-SSCP和DNA测序技术研究了187头纯种南阳牛Gli3基因编码区4个位点(E4、E5、E6和E7)的多态性,发现E4和E5位点存在SSCP多态。对不同SSCP带型的对应片段进行了测序分析,共发现3个新的SNP多态位点(T123600C、T123696C和A128688G),其中第123600和第123696两个位点处于连锁状态。E5位点处于Hardy-Weinberg平衡状态,E4位点处于非平衡状态。不同基因型与生长发育性状的相关性分析显示,E4位点上,CC基因型个体的六月龄体重、胸围、坐骨端宽、日增重指标显著大于TT基因型个体(P<0.05),该位点有可能作为南阳牛早期生长性状标记辅助选择的标记之一。As a member of Gli-Kruppel family, Gli3 can regulate the Sonic hedgehog target genes in the growth and development of animal, especially in the early time. In this study, polymorphisms of four loci（E4, E5, E6 and E7） at the coding region of the bovine Gli3 gene were detected in 187 Nanyang bovine by PCR-SSCP and sequencing method. The fragments showing different SSCP patterns were sequenced, and a total of three SNPs （T123600C, T123696C and A128688G） were found. The E5 locus of Gli3 gene was equilibrium at Hardy-Weinberg, while E4 locus of Gli3 gene was not disequilibrium at Hardy-Weinberg. The association analysis of different genotypes with growth traits showed that the individuals with genotype CC had a larger body weight, chest girth, hucklebone width, average daily gain than that of the individuals with genotypes TT（P0.05） at six months in E4 locus. It suggests that E4 locus may be one of the marker-assisted selection used as growth traits in Nanyang bovine.

关 键 词：Gli3 南阳牛 PCR-SSCP 生长性状 

分 类 号：S828.2[农业科学—畜牧学]