串联抑制素基因免疫对绵羊生殖的影响  

Effect of Tandem Inhibin Gene Immunization on Reproduction of Sheep

作　　者：郭宪[1,2] 岳耀敬[2] 焦硕[2] 冯瑞林[2] 张勇[1] 赵兴绪[1] 

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]中国农业科学院兰州畜牧与兽药研究所,兰州730050

出　　处：《农业生物技术学报》2010年第6期1143-1148,共6页Journal of Agricultural Biotechnology

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(NoBRF060104)资助

摘　　要：利用基因免疫技术,以成功构建的串联抑制素重组质粒pcDNA-DPPISS-DINH和pcDNA-DPPISS-DINH-sC3d3为免疫原,对60只绵羊(Ovisaries)进行基因免疫试验;应用酶联免疫法(ELISA)检测抗抑制素抗体;放射免疫法(RIA)测定处理后不同时期血清中激素水平。结果表明,重组质粒pcDNA-DPPISS-DINH和pcDNA-DPPISS-DINH-sC3d3免疫绵羊后,各实验组P(血样吸光值)/N(对照吸光值)值均显著高于对照组(P<0.05),且在第3次加强免疫后抗体水平明显上升。首次免疫后,促卵泡素(FSH)平均含量均高于对照组,且在第2次、第3次加强免疫阶段差异显著(P<0.05)。第2次免疫后促黄体素(LH)、雌二醇(E2)和孕酮(P)含量均高于对照组(P>0.05),第3次加强免疫后E2和P含量均显著高于对照组(P<0.05)。两种重组质粒免疫绵羊后,双羔率分别为22.2%和30.0%,均与对照组差异显著(P<0.05)。结果提示,串联抑制素基因免疫绵羊可促进FSH分泌,分子佐剂sC3d能增加绵羊对串联抑制素基因免疫的反应性,进而影响绵羊卵泡发育和诱导绵羊产双羔。Using the technology of gene immunization, 60 sheep（Ovis aries） were immunized with recombinant plasmid of tandem inhibin gene, pcDNA-DPPISS-DINH and pcDNA-DPPISS-DINH-sC3d3, respectively. The P（Positive absorbancy of blood sera）/N（Negative absorbancy of control） values of antibody against inhibin were detected by ELISA（enzyme-linked immunoassay） and the hormone levels were detected at different stage by RIA（radioimmunoassay） after gene immunization of tandem inhibin in sheep. The results showed that the P/N values of antibody against inhibin in gene immunization groups of pcDNA-DPPISS-DINH and pcDNA-DPPISS-DINH-sC3d3 were higher than those in control group（P0.05）, respectively, and the levels of antibody against inhibin were rose significantly after the third immunization. The levels of follicle stimulating hormone（FSH） in gene immunization groups were higher than those in control group after the first immunization, and the difference was significant after the second and third immunization （P0.05）, respectively. The levels of LH（luteinizing hormone）, E2（estradiol） and P（progesterone） were all higher than those in control group after the second immunization, but there were no significant differences （P0.05）. After the third immunization, the levels of E2 and P were higher than those in control group（P0.05）, and the twinning rates were 22.2% and 30.0%, respectively, which were significantly higher（P0.05） than that in the control group. All the results showed that the recombinant plasmid of tandem inhibin gene as vaccine for sheep can stimulate FSH secretion, and molecular adjuvant sC3d（sheep complement 3d） can promote immune response, so as to effect follicle development and induce sheep twinning.

关 键 词：串联抑制素 基因免疫 生殖激素 双羔率 绵羊 

分 类 号：S852.4[农业科学—基础兽医学]