一种适用于甘蔗SSR-PCR的DNA快速提取方法  

A rapid DNA extraction method from sugarcane leaves for SSR-PCR

作　　者：陈忠良[1,2,3] 秦翠鲜[1,4] 郭元元[1,4] 杨翠芳[1,4] 罗海斌[1,4] 何海波[1,4] 

机构地区：[1]广西作物遗传改良生物技术重点开放实验室,南宁530007 [2]农业部甘蔗品质监督检验测试中心(南宁) [3]广西甘蔗遗传改良重点实验室,南宁530007 [4]广西师范大学生命科学学院,广西桂林541004

出　　处：《广西农业科学》2010年第11期1155-1157,共3页Guangxi Agricultural Sciences

基　　金：国家科技支撑计划项目(2007BAD30B05);广西自然科学基金项目(桂科自0991183);广西科学研究与技术开发计划项目(桂科攻0782004-5,桂科能0815011-6-1);广西农业科学院基本科研业务专项项目(200803Z-基)

摘　　要：以不同甘蔗品种幼苗期幼嫩叶片为材料,以改良SDS法和DNA快速提取法对甘蔗基因组DNA进行提取,并以SSR-PCR检测比较两者的扩增效果。结果表明,两种方法提取获得的基因组DNA条带基本一致,条带较为清晰,重复性好且稳定,均可满足SSR-PCR扩增的需要。但DNA快速提取法简化了SDS改良法,具有高通量、操作方便、简单、快速、成本低、DNA存放时间较长等优点,能在较短时间内完成大量样品的DNA提取。The young leaves of different sugarcane varieties were sampled at settling stage to extract genomic DNA,and a rapid DNA extraction method was developed by modifications in SDS method.The extracted DNA was amplified in PCR using SSR primers.The results showed no differences in the amplified bands of the DNA isolated using the two different methods.The DNA isolated from rapid method showed clear and reproducible bands,and was found suitable for the application in marker related studies.Further,the DNA isolated using the rapid extraction method had advantages over the other method in terms of high throughput,rapidity,easy and simple operation,low cost and longer storing time.Using this method,DNA can be isolated from a large number of sugarcane leaf samples within a short time.

关 键 词：甘蔗 DNA提取 SDS改良法 DNA快速提取法 

分 类 号：S566.1[农业科学—作物学]