脑得生有效部位组方中葛根素在兔体内的药动学  

Pharmacokinetics of puerarin from Naodesheng composed of active fractions in rabbit

作　　者：王光忠[1] 肖锦[1] 樊文娟[1] 刘焱文[1] 

机构地区：[1]湖北中医药大学药学院,湖北武汉430065

出　　处：《中国医院药学杂志》2010年第24期2067-2070,共4页Chinese Journal of Hospital Pharmacy

基　　金：湖北省自然科学基金项目(编号:2008CDB234)

摘　　要：目的:以脑得生的有效部位组方,比较脑得生有效部位方与脑得生原方中葛根素在兔体内的生物利用度。方法:分别提取脑得生的有效部位,并以有效部位配伍组方。将实验大白兔分成2组,分别灌胃脑得生的有效部位和脑得生粗提物,采用高效液相色谱法测定不同时间兔血浆中葛根素浓度,应用PKSolver2.0软件计算药动学参数。结果:脑得生有效部位与脑得生粗提物的主要药动学参数:AUC0-4分别为(6.0±3.1)mg.h.L-1和(5.7±3.4)mg.h.L-1,Cmax分别为(3.1±0.9)mg.L-1和(2.87±0.10)mg.L-1,tmax分别为(0.72±0.20)h和(0.73±0.10)h,两者无明显差异,相对生物利用度为(106.7±10.0)%。结论:脑得生由其有效部位组方,口饲后,其活性成分的吸收和消除并未发生变化,表现出与脑得生粗提物相同的作用。OBJECTIVE To study the pharmacokinetics of puerarin in Naodesheng composed of active fractions. METHODS The fomulation was composed of active fractions purified from Naodesheng. Compared with the coarse extracts of Naodesheng,the concentration of puerarin in serum of rabbit and their pharmacokinetics after oral administration were studied by HPLC. The pharmacokinetic parameters were computed by software program PKSolver 2. 0. RESULTS The main pharmacokinetic parameters of Naodesheng composed of active fractions and the coarse extracts of Naodesheng were： AUG,-4 （6. 0 ± 3. 1 ）mg·h·L^-1 and （5.748 ± 3. 4）mg·h·L^-1,Cmax（3.1 ± 0. 9）mg·L^-1 and （2.87 ±0. 1mg·L^-1 ,tmax（0.72± 0. 20）h and （0.73 ± 0. 10）h,respectively. The differences of the major pharmacokinetic parameters between test and reference were not significant. The relative bioavailability of test was （106. 7 ± 10. 0） %. CONCLUSION Bioavailability of puerarin in Naodesheng composed of active fractions is in accordance with that of the coarse extracts after oral administration.

关 键 词：脑得生有效部位方 葛根素 药动学 生物利用度 

分 类 号：R969.1[医药卫生—药理学]