毛细管电泳-激光诱导荧光检测法测定抗癫痫药加巴喷丁  

Determination of gabapentin using capillary electrophoresis with laser-induced fluorescence detection

作　　者：蔡元丽[1] 杨甲甲[1] 王宇飞[1] 白珂珂[1] 李晖[1] 

机构地区：[1]四川大学化学工程学院,四川成都610065

出　　处：《色谱》2010年第12期1179-1184,共6页Chinese Journal of Chromatography

摘　　要：建立了毛细管电泳-激光诱导荧光(CE-LIF)测定抗癫痫药加巴喷丁的方法。加巴喷丁经4-氯-7-硝基苯并-2-氧杂-1,3-二唑(NBD-Cl)衍生化后,采用10mmol/L硼砂-10mmol/L十二烷基硫酸钠(pH9.75)的缓冲体系,加巴喷丁在6min内实现高效基线分离。方法的线性范围为0.01～10mg/L(r=0.999 7),检出限为2μg/L,定量限为10μg/L。方法的平均回收率为100.2%～103.1%,相对标准偏差为0.15%～1.00%(n=3)。该方法灵敏、快速、准确和可靠,已用于加巴喷丁药物制剂的质量控制。A novel method for the determination of gabapentin（an antiepileptic drug） has been developed using capillary electrophoresis with laser-induced fluorescence detection（LIFD）.Gabapentin was first derived by 4-chloro-7-nitrobenzofurazan（NBD-Cl） and baseline separated within 6 min with a running buffer consisting of 10 mmol/L borate sodium and 10 mmol/L sodium dodecyl sulfate（pH 9.75）.The linear range of this method was 0.01-10 mg/L（r=0.999 7） with the limit of detection（LOD） of 2 μg/L and the limit of quantification（LOQ） of 10 μg/L.The average recoveries were 100.2%-103.1% with the relative standard deviation（RSD） of 0.15%-1.00%.This method is fast,sensitive,accurate and reliable,and it has been used successfully for the quality assessment of gabapentin drug products.

关 键 词：毛细管电泳-激光诱导荧光检测 加巴喷丁 4-氯-7-硝基苯并-2-氧杂-1 3-二唑 抗癫痫药 

分 类 号：O658[理学—分析化学]