艾灸血浆对乙醇损伤的人胃黏膜上皮细胞的影响  

Plasma of individuals undergoing moxibustion has protective effects against alcohol-induced injury to human gastric epithelial cells in vitro

作　　者：洪金标[1,2] 易受乡[1] 黄芸[1] 林亚平[1] 杜燕[1] 彭宏[1] 彭艳[1] 

机构地区：[1]湖南中医药大学针灸推拿学院经穴与脏腑相关重点研究室,湖南省长沙市410007 [2]深圳市中医院推拿科,广东省深圳市518033

出　　处：《世界华人消化杂志》2010年第31期3287-3293,共7页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30772707;教育部博士点科学基金资助项目;No.20070541003~~

摘　　要：目的:探讨艾灸血浆对乙醇损伤的离体人胃黏膜上皮细胞(GES-1)的保护作用.方法:将离体胃黏膜上皮细胞(GES-1)分为4组,即空白组、乙醇损伤模型组、艾灸穴位血浆组和艾灸非穴位血浆组.艾灸人体足三里、中脘、关元穴及非穴对照点前后提取并制备艾灸前后的血浆.以含8%乙醇培养液作用于后3组胃黏膜上皮细胞,建立胃黏膜上皮细胞损伤模型.各组分别加入预先提取的灸前血浆、艾灸穴位血浆和艾灸非穴位血浆.采用MTT法检测细胞抑制率,比色法检测细胞培养上清液中液乳酸脱氢酶(lactate dehydrogenase,LDH)、丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)的含量,流式细胞技术检测细胞周期及细胞凋亡率.结果:模型组与空白组比较,细胞A490降低(P<0.01),抑制率明显上升;与模型组比较,艾灸穴位血浆组A490升高(P<0.01),细胞抑制率降低;艾灸非穴位血浆组A490低于艾灸穴位血浆组A490(P<0.01),而细胞抑制率高于艾灸穴位血浆组(26.52%vs22.06%).与空白组比较,模型组培养液中LDH、MDA、SOD含量均有所提高(P<0.01或0.05);艾灸穴位血浆组与模型组比较,SOD水平升高(P<0.01),LDH、MDA含量降低(均P<0.01);艾灸非穴位血浆组与艾灸穴位血浆组比较,SOD水平下降(34.11kU/L±4.14kU/Lvs38.73kU/L±4.34kU/L,P<0.05),LDH和MDA水平升高(9.26U/L±1.62U/Lvs7.38U/L±1.28U/L;3.91μmoL/L±0.11μmoL/Lvs3.75μmoL/L±0.11μmoL/L,均P<0.05).艾灸非穴血浆组与艾灸穴位血浆组比较,细胞周期中各期无明显差异(均P>0.05),凋亡率升高(1.56%±0.19%vs1.24%±0.08%,P<0.01).结论:艾灸足三里、中脘、关元穴后提取的人体血浆对受损人胃黏膜细胞具有一定的保护作用.AIM：To investigate whether plasma of individuals undergoing moxibustion has protective effects against ethanol-induced injury to human gastric epithelial cells（GES-1）.METHODS：Isolated GES-1 cells were divided into four groups：control group,ethanol injury group,test plasma group（plasma from individuals undergoing moxibustion at specific points）,and control plasma group（plasma from individuals undergoing moxibustion at non-specific points）.Before and after moxibustion at Zusanli,Zhongwan,Guanyuan or non-specific points,blood plasma was prepared.Cells of the ethanol injury group,test plasma group,and control plasma group were treated with 8% ethanol to induce cell injury.After injured cells were incubated with plasma,cell growth was measured by MTT assay;the contents of LDH,SOD and MDA in culture supernatants were determined by colorimetric assay;and cell cycle progression and apoptosis were detected by flow cytometry.RESULTS：Compared with the control group,A490 value significantly decreased（P〈0.01） and the reduced rate of cell growth increased in the ethanol injury group.Compared with the ethanol injury group,both A490 value（P〈0.01） and the reduced rate of cell growth decreased significantly in the test plasma group.The reduced rate of cell growth was significantly higher in the control plasma group than in the test plasma group（26.52% vs 22.06%）.Compared with the control group,the contents of LDH,MDA and SOD increased in the ethanol injury group（all P〈0.01 or 0.05）.Compared with the ethanol injury group,an increase in SOD content（P〈0.01） and a decrease in LDH and MDA contents（both P〈0.01） were noted in the test plasma group.SOD content decreased（34.11 kU/L ± 4.14 kU/L vs 38.73 kU/L ± 4.34 kU/L,P〈0.05） and LDH and MDA levels increased（9.26 U/L ± 1.62 U/L vs 7.38 U/L ± 1.28 U/L;3.91 μmoL/L ± 0.11 μmoL/L vs 3.75 μmoL/L ± 0.11 μmoL/L,both P〈0.05） in the control plasma group compared with the test plasma group.No signifi cant difference was not

关 键 词：艾灸血浆 人胃黏膜上皮细胞 细胞凋亡 乳酸脱氢酶 丙二醛 超氧化物歧化酶 

分 类 号：R246.1[医药卫生—针灸推拿学]