土壤中棉秆纤维素降解菌的筛选  

Isolation of Cotton Straw Cellulose-degrading Fungi from Soil

作　　者：高静[1] 席琳乔[1] 马春晖[1] 

机构地区：[1]塔里木大学动物科学学院/塔里木畜牧科技兵团重点实验室,新疆阿拉尔843300

出　　处：《塔里木大学学报》2010年第4期6-10,共5页Journal of Tarim University

基　　金：国家自然科学基金(30960267)

摘　　要：本研究应用纤维素刚果红培养基从棉田土壤中筛选纤维素降解菌株,通过CMC酶活、FPA和实际降解效率的测定,筛选出5株能分解纤维素的菌株,分别编号为T4、T3、T2、M4、M3。结果表明,通过对培养和培养温度的优化,发现在培养时间为72 h、培养温度为30℃时纤维素分解酶的活性最佳。在第5 d、10 d、15 d、20 d测定菌株对棉花秸秆中纤维素和木质素均有一定的降解能力,在培养20 d时,对纤维素和木质素的降解速率分别为T4 18.62%、14.2%;T3 18.27%、12.97%;T2 17.53%、13.54%;M4 19.69%、13.56%;M3 19.21%、14.25%。Cellulose congo-red medium was used to isolate strains of cellulose-decomposing from soil in the study.According to the detection of CMC activity 、FPA activity and the efficiency of the actual degradation,values 5 strains which were screened of cellulose-decomposing microorganisms are numbered T4,T3,T2,M4,M3.The results showed that the culture time at 72 h and temperature at 30 ℃ was the best culture condition for cellulose-decomposing enzyme by the optimization of culture time and temperature value.And at 5,10,15,20 days,the degradative ability of cellulose and lignin of cotton stalk was determined.At 20 days,the rates of degradation to cellulose and lignin were T4 18.62%,14.2%;T3 18.27%,12.97%;T2 17.53%,13.54%;M4 19.69%,13.56%;M3 19.21%,14.25%.

关 键 词：棉花秸秆 菌株 木质素 纤维素 

分 类 号：S816[农业科学—饲料科学]