玻璃基底表面抗蛋白吸附膜的制备及性能测试  

Fabrication and Performance Testing of Various Protein-Resistant Films on the Glass Substrates

作　　者：余海[1] 黄宁平[1] 

机构地区：[1]东南大学生物科学与医学工程学院生物电子学国家重点实验室,江苏南京210096

出　　处：《化工时刊》2010年第12期7-10,共4页Chemical Industry Times

基　　金：国家自然科学基金面上项目(30870626)

摘　　要：通过分子自组装技术和原子转移自由基聚合方法(ATRP)在玻璃基底表面分别制备了不同的抗蛋白吸附膜,采用接触角测量,光波导模式谱仪和荧光标记蛋白的吸附对膜表面性能进行了测试。结果表明,两种方式形成的3种抗蛋白吸附膜能够阻止绝大部分的蛋白吸附,其中ATRP方式形成的聚乙二醇(PEG)膜表面其抗蛋白吸附性能略优于自组装膜,而磺酸基甜菜碱—异丁烯酸(SBMA)形成的聚合物刷相比于PEG具有更好的抗蛋白吸附能力。The protein-resistant films on the glass substrates were fabricated via molecular self-assembly and atom transfer radical polymerization（ATRP）,respectively.The resulting films were characterized by water contact angle measurements,optical waveguide lightmode spectroscopy（OWLS）,and the adsorption of FITC-labeled protein.The results indicated that the fabricated protein-resistant films indeed repelled most of protein adsorption,which could serve as the optimal interfaces for most bio-electronic devices.The quantitative results from OWLS measurements further indicated that the polymer brushes formed by ATRP is superior to the corresponding self-assembled films in terms of the protein-resistant property.Additionally,the poly（sulfobetaine methacrylate）（polySBMA） brushes exhibited even better protein resistance compared to the poly（ethylene glycol） methacrylate（PEGMA） brushes.

关 键 词：玻璃基底 抗蛋白吸附膜 分子自组装 原子转移自由基聚合 

分 类 号：TB43[一般工业技术]