长春花脱乙酰氧基文多灵-4-羟化酶基因启动子的克隆与生物信息学分析  

Molecular Cloning and Analysis of Deacetoxyvindoline 4-Hydroxylase Promotor in Catharanthus Roseus(L.) G. Don

作　　者：周晨[1] 赵淑娟[1] 胡之璧[1] 

机构地区：[1]国家中医药管理局中药资源与质量标准重点研究室上海市复方中药重点实验室上海中医药大学中药研究所,上海201203

出　　处：《药物生物技术》2010年第6期471-477,共7页Pharmaceutical Biotechnology

基　　金：国家自然科学基金(编号:30200358);教育部高等学校博士学科点专项科研基金

摘　　要：通过基因组步行技术克隆到长春花脱乙酰氧基文多灵-4-羟化酶基因上游905bp启动子序列,生物信息学分析发现该序列中存在多个与光照诱导有关的顺式元件,同时也发现一个与长春花特异的顺式作用元件JERE类似的短序列,推测该序列是茉莉酸诱导途径中转录因子ORCA3的特异结合位点。Desacetoxyvindoline-4-hydroxylase（D4H） is an important enzyme in the biosynthetic pathway of monoterpenoid alkaloids in Catharanthus roseus.It catalyzes the 4-hydroxylation of desacetoxyvindoline to deacetylvindoline.The aim of this work was to clone and to investigate the functional profiles of d4h promoter.The C.roseus d4h promoter sequence was isolated by a PCR-based genome walking method.As a result,a 905bp promotor sequence was cloned based on the cDNA sequence of d4h from Genbank.Sequence analysis using PlantCARE online database showed several cis-acting elements,including several light-induction-involving elements.It demonstrates the mechanism of the light to vindoline biosynthesis.In addition,a short region similar to the C.roseus cis-element JERE was also found.We speculate that this region may be the binding site of the C.rosues transcription factor ORCA3.

关 键 词：长春花 文多灵 脱乙酰氧基文多灵-4-羟化酶 启动子 染色体步行 

分 类 号：S567[农业科学—中草药栽培]