合欢皮总皂苷急性毒理学研究  

Research on Emergency Toxicology of Total Saponin in Silktree Albizia Bark

作　　者：赵建国 刘玲艳 朱颖越 邱丽颖 冯磊 金坚 

机构地区：[1]江南大学医药学院,无锡214122

出　　处：《天然产物研究与开发》2010年第4期582-586,共5页Natural Product Research and Development

基　　金：国家自然科学基金项目(30772586)

摘　　要：本文采用常规急性毒理学研究的方法探究合欢皮总皂苷对小鼠的毒性剂量。HE染色观察其主要器官形态学变化,玻璃毛细管法测定血液凝固时间,生化法测定超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-PX)活性。合欢皮总皂苷的最小致毒量为7.5 mg/kg,近似致死量为1500 mg/kg,半数致死量(LD50)为2164 mg/kg。毒性表现为动物活动减少、1500 mg/kg以上剂量使小鼠食欲下降、2250 mg/kg剂量使小鼠体重明显下降;给药后1 d、7 d和14 d给药组血液凝固时间缩短;主要器官形态学明显改变,明显降低主要器官SOD和GSH-PX活性。结果表明合欢皮总皂苷对小鼠有一定的毒性作用。We used the routine researching methods of acute toxicology to determine the toxic dose of total saponin in silktree albizia bark.The morphological change of major organs was observed by HE staining.The coagulation time was determinated by glass capillary method.And the SOD and GSH-PX activity were measured by biochemical method.This paper indicates that the minimum toxic dose of total saponin of silktree albizia bark is 7.5 mg/kg,the approximate lethal dose is 1500 mg/kg and the median lethal dose is 2164 mg/kg.In this research the mice had little action.The dose of total saponin above 1500 mg/kg decreased the appetite of mice.When the dose reached 2250 mg/kg,the body weight of mice decreased obviously.After having been given drugs for 1 day,7 day,or 14 day,the blood coagulation time of mice was shortening.The morphosis of main organs in mice were abnormal,and the activity of superoxide dismutase（SOD） and glutathione peroxidase（GSH-PX） of the main organs were decreased after given the saponin of silktree albizia bark.The results showed that the total saponin of silktree albizia bark had significant toxic effect on mice after being treated more than 7.5 mg/kg of dosage.

关 键 词：合欢皮 总皂苷 毒性 致毒剂量 小鼠 

分 类 号：R285.6[医药卫生—中药学] Q946.91[医药卫生—中医学]