木荷及其与无患子皂甙抽提物单复配体外抗稻瘟病活性(英文)  

In vitro Antifungal Activity of Saponin Extracts from Schima superba in Combination with Sapindus mukorossi against Piricularia oryzae

作　　者：霍光华[1] 詹五根[1] 付日辉[1] 陈明辉[1] 

机构地区：[1]江西农业大学生物科学与工程学院,南昌330045

出　　处：《天然产物研究与开发》2010年第5期755-760,共6页Natural Product Research and Development

基　　金：supported by Science Project from Jiangxi Education Department(GJJ09162);Jiangxi Natural Science Fund(2009GZN0029);Chinese National Natural Science Fund(31060250)

摘　　要：来自木荷叶和无患子中果皮的皂甙抽提物对抗稻瘟病原活性被试验。皂甙抽提物体外抑制活性显示了皂甙剂量与抗真菌效果高度相关性。它们的抗稻瘟有效中浓度EC50:木荷叶乙醇抽提物为42.95μg/mL,木荷叶水抽提物为452.91μg/mL,无患子中果皮的甲醇抽提物为95.65μg/mL。一个重要的结果是当上述木荷叶和无患子中果皮醇抽提物以3∶4到15∶8的质量配比时产生了显著的增效抗稻瘟作用,而在其它配比时有相加作用。通过乙醚/丙酮沉析、硅胶柱色谱分离,以及酸水解和薄层过程,检测出木荷抽提物皂甙中含有2种三萜类皂甙元和5种单糖。纯化后的皂甙在150μg/mL时显示了98.28%的抑制率。此研究结果表明:木荷叶抽提物,或木荷叶和无患子中果皮抽提复配物对毁灭性的稻瘟病害具有重要地抗病效果。Saponin extracts from Schima superba leaves（SSL） and Sapindus mukorossi fruit mesocarp（SMFM） have been tested against the pathogen Piricularia oryzae.The inhibitory effect of these extracts measured in vitro show a nearly relationship between the saponin dose and the antifungal effect.Their values of EC50（effective concentrations at 50% inhibitory rate） are 42.95 μg/mL for an ethanolic extract of SSL,452.91 μg/mL for a water extract of SSL and 95.65 μg/mL for a methanolic extract of SMFM respectively.An important result was that combination of extracts from SSL and SMFM have notable synergistic effect at ratios ranged from 3∶4 to 15∶8（m/m） and additive effect at other ratio.In addition,antifungal components of SSL were established as triterpenoid saponins composed of 2 aglycones and 5 monosaccharides after purification by ethyl ether/acetone precipitation,silica gel chromatography,hydrolysis and TLC procedure.The purified saponin complex showed up to a 98.28% inhibitory rate at 150 μg/mL.The results suggested that selected extracts from SSL or a combination of SSL and SMFM can play an important role in resistance Piricularia oryzae,a destructive disease of rice.

关 键 词：皂甙抽提物 复配 木荷 无患子 稻瘟病 

分 类 号：S482.292[农业科学—农药学]