滇黄芩再生植株中黄酮类化合物的组织化学定位及黄芩苷的含量测定  

Histochemical Localization of Flavonoids and Determination of Baicalin in Regenerated Plants of Scutellaria amoena C.H.Wright

作　　者：岑举人[1,2] 步怀宇[1] 孔祥鹤[1] 王英娟[1] 贾敬芬[1] 

机构地区：[1]西北大学西部资源生物与现代生物技术教育部重点实验室,西安710069 [2]北京师范大学生命科学学院,北京100875

出　　处：《天然产物研究与开发》2010年第6期1069-1072,共4页Natural Product Research and Development

基　　金：陕西省自然科学基金(SJ08B05);陕西省教育厅基金(09JS080)

摘　　要：应用植物组织化学定位方法,研究了组织培养滇黄芩再生植株中黄酮类化合物的积累分布状态;并且通过HPLC对滇黄芩再生植株中黄芩苷的含量进行测定。结果表明,滇黄芩再生植株黄酮类化合物主要存在于营养器官的表皮和皮层,以及茎、叶腺毛中。同时,测定再生植株中黄芩苷的含量表明,地下部分(根)含量为0.12%;地上部分(茎、叶)含量为0.43%。The histochemical method was applied in the study of the localization of flavonoids in the regenerated plantlets of Scutellaria amoena C.H.Wright.The results showed that the flavonoids were mainly distributed in the epidermis and cortex parenchyma of the vegetative organs.The flavonoids were also found to accumulate in the tentacles of the stems and leaves.In addition,the content of baicalin in the regenerated plantlets was determined by HPLC.The content of baicalin in the stems and leaves（0.43%）was higher than that in the roots（0.12%）.

关 键 词：滇黄芩 再生植株 组织化学 黄酮类化合物 黄芩苷 HPLC 

分 类 号：Q94-334[生物学—植物学] R284.2[医药卫生—中药学]