浙江省不同地区实验兔血液蛋白多态性研究  

Serum Protein Loci Polymorphism in Laboratory Rabbits Bred in Different Regions of Zhejiang Province

作　　者：赵伟春[1] 徐孝平[2] 李莉[1] 王德军[2] 喻琦[3] 陈民利[2] 

机构地区：[1]浙江中医药大学生物工程学院,杭州310053 [2]浙江中医药大学动物实验研究中心,杭州310053 [3]浙江工业大学药学院,杭州310014

出　　处：《中国实验动物学报》2010年第6期506-513,共8页Acta Laboratorium Animalis Scientia Sinica

基　　金：浙江省科技厅实验动物公共服务平台项目(No.2008F80017;No.Dwpt-bz-12);浙江省卫生高层次创新人才培养工程项目(2008年)

摘　　要：目的研究浙江省不同产地实验兔血液蛋白的多态性,了解各地实验兔的遗传背景,指导优良品系的选育。方法选择兰溪、农科院、上虞和余姚等地生产的实验兔的11个血液蛋白位点进行聚丙烯酰胺凝胶电泳,根据电泳图谱分析基因频率、Hardy-weinberg平衡状态、遗传变异程度和遗传相似性。结果 Pr、Ptf、Hbα、Hbβ和Alb在所有实验兔中表现为单态,其余蛋白位点呈多态。各蛋白位点的变异程度Prt1>Sag>Tf1>Po>Tf2>Prt2。各地实验兔蛋白位点平均杂合度为兰溪>农科院>上虞>余姚。农科院与余姚实验兔间的遗传距离最近,上虞与兰溪实验兔的亲缘关系最远,遗传相似系数分别为0.7938和0.7687。结论血液蛋白多态性反映了浙江省不同地区生产的实验兔的遗传背景,为实验兔的选育提供了实验依据。Objective To study the serum protein loci polymorphism in rabbits bred in different regions of Zhejiang province.Method Eleven blood protein loci of rabbits bred in four regions of Zhejiang province were analyzed by vertical polyacrylamide gel electrophoresis.The rabbits were bred in the Agriculture Academy,in Lanxi,Shangyu and Yuyao,respectively.The proteins included the prealbumin（Pr）,postalbumin（Po）,pretransferrin 1（Prt1）,pretransferrin 2（Prt2）,transferrin 1（Tf1）,transferrin 2（Tf2）,posttransferrin（Ptf） and slow α-globulin（Sag）,respectively.The gene frequency,Hardy-Weinberg balance,genetic variation and similarity coefficient of the four groups of rabbits were analyzed.Results Pr,Ptf,Hbα,Hbβ and Alb in all rabbits had a monomorphism,while the others were polymorphic.The genetic variation of serum protein loci were Prt1 Sag Tf1 Po Tf2 Prt2.The heterozygosity of serum protein loci were Lanxi Agriculture Academy Shangyu Yuyao.The genetic distance between the rabbits bred in Agricultural Academy and Yuyao was the nearest among those four regions.The similarity coefficient was 0.7938.The relationship between the rabbits bred in Shangyu and Lanxi was the farthest,with a similarity coefficient of 0.7687.Conclusions The serum protein polymorphism reflects the genetic background of the rabbits bred in different regions of Zhejiang province.It provides an experimental basis for breeding of rabbits.

关 键 词：兔 血液蛋白多态性 基因频率 杂合度 遗传距离 

分 类 号：Q346[生物学—遗传学]