重组质粒pDsRed1-C1-TRAIL的构建及鉴定  

Construction and identification of recombinant plasmid of pDsRed1-C1-TRAIL

作　　者：孙念峰[1] 孟庆义[1] 田爱玲[1] 王瑞华[1] 刘兆轩[1] 李博[1] 

机构地区：[1]山东大学附属济南市中心医院普外科,山东济南250013

出　　处：《中国现代普通外科进展》2010年第11期849-851,857,共4页Chinese Journal of Current Advances in General Surgery

基　　金：教育部新教师基金(200804221081);山东省自然科学基金(Y2008C08)

摘　　要：目的:构建重组质粒pDsRed1-C1-TRAIL并鉴定,为下一步利用纳米载体介导FL基因移植作用于结肠癌细胞的体内实验奠定基础。方法:应用基因合成和克隆技术构建重组质粒pDsRed1-C1-TRAIL,并进行酶切鉴定及序列测定,以检测其核苷酸序列与设计是否完全一致。结果:重组质粒pDsRed1-C1-TRAIL经酶切鉴定分析,琼脂糖凝胶电泳鉴定可见清晰地切出与TRAIL基因大小相符的片段并与DNA marker相符,同时进行序列测定,证实其核苷酸序列与设计完全一致。结论:成功构建重组质粒pDsRed1-C1-TRAIL并得到正确鉴定,为下一步结肠癌的基因治疗打下实验基础。Objective： To construct and identify recombinant plasmid of pDsRed1-C1-TRAIL,which was then transfected into colon cancer cells.Methods： Recombinant plasmid of pDsRed1-C1-TRAIL was constructed and identified by using genes synthesis and cloning technique.The cloned fragment was sequenced and identified by restriction enzyme.Results： Recombinant plasmid of pDsRed1-C1-TRAIL was identified successfully by restriction enzyme.Meanwhile,the nucleotide sequence was confirmed that it matched with design completely by sequencing.Conclusion： Recombinant plasmid of pDsRed1-C1-TRAIL is constructed successfully which lays the foundation for further study on gene therapy of colon cancer.

关 键 词：重组质粒 TRAIL基因 基因治疗 

分 类 号：R687[医药卫生—骨科学]