弓形虫感染对胎鼠铁含量和胎盘铁转运蛋白表达的影响  

Changes in iron concentrations in fetal mice and expression of iron transport proteins in the placenta during pregnancy as a result of infection with Toxoplasma gondii

作　　者：赵晋英[1] 黄泽智[1] 李艳伟[1] 侯玉英[2] 王秀虎[1] 何跃华[1] 孙友平[1] 

机构地区：[1]湖南省邵阳医学高等专科学校检验系,湖南邵阳422000 [2]山西医科大学寄生虫学教研室,山西太原030001

出　　处：《中国病原生物学杂志》2010年第12期904-907,F0003,共5页Journal of Pathogen Biology

基　　金：湖南省科技厅科技计划项目(No.2009FJ3065)

摘　　要：目的探讨刚地弓形虫感染孕鼠后对胎鼠体内铁（Fe）含量和胎盘铁转运蛋白表达的影响。方法孕8 dBALB/c小鼠经腹腔接种弓形虫速殖子,在孕10、12、14、16和18 d剖腹,取胎鼠,采用原子吸收分光光谱法测定胎鼠体内铁含量;用DAB增强的铁染色法观察胎鼠组织中铁分布;用免疫组织化学染色法检测胎盘膜铁转运蛋白（FP1）和铜蓝蛋白（Cp）的表达。结果在孕10 d,感染组胎鼠体内铁含量与对照组比较差异无统计学意义（P〉0.05）;孕12~18d,铁含量均低于对照组（P〈0.05）。感染组胎鼠整体、胎肝及胎脑组织铁染色强度与对照组相比显著减弱。FP1和Cp主要表达于胎盘合体滋养层细胞（STB）基底侧,在孕10、12 d,感染组FP1和Cp与对照组比较差异无统计学意义（P〉0.05）;在孕14、16和18 d,感染组FP1和Cp表达显著高于对照组（P〈0.05）。结论小鼠孕期感染弓形虫可使胚胎铁含量降低,并影响胚胎的生长发育;胎盘FP1和Cp高表达可能是胎鼠体内铁缺乏的代偿反应;胎盘FP1和Cp的表达可能受胎鼠体内铁浓度的调控。Objective To study the changes in iron concentrations in fetal mice and expression of iron transport proteins in the placenta during pregnancy as a result of infection with Toxoplasma gondii.Methods Experimental models were created by intraperitoneal injection of tachyzoites of T.gondii on the 8th day of pregnancy.The concentration of Fe in fetal mice was determined by flame atomic absorption spectrometry（FAAS）.Iron in fetal mice was stained by DAB-enhanced Perls＇ staining.The expression of ferrportin1（FP1） and ceruloplasmin（Cp） in the placenta was detected immunohistochemically.Results On the 10th day of pregnancy,there was no significant difference in the Fe concentration in fetal mice for the two groups,but the Fe concentration in the experimental group was significantly lower than that in the control group on the 12-18th day of pregnancy（P0.05）.Iron staining of the entire fetal mouse and liver and brain of fetal mice in the infected group was significant weaker than that in the control group.FP1 and Cp were expressed in the basal（fetal facing） membrane of the placental syncytiotrophoblast（STB）,consistent with unidirectional iron transport from the mother to the fetus.On the 10th and 12th day of pregnancy,there were no significant differences in the expression of FP1 and Cp in the placenta for the two groups（P0.05）.However,the expression of FP1 and Cp in the placenta of the experimental group was significantly higher than that in the control group on the 14-18th day of pregnancy（P0.05）.Conclusion Infection with T.gondii during pregnancy induced a decrease in the fetal iron concentration,and this phenomenon might be involved in the retardation of fetus growth.The increased expression of FP1 and Cp in the placenta may be the result of compensating for decreased fetal iron.This is indirect evidence that the expression of FP1 and Cp in the placenta might be regulated by fetal iron concentration.

关 键 词：刚地弓形虫 铁 胎盘 膜铁转运蛋白 铜蓝蛋白 

分 类 号：R382.5[医药卫生—医学寄生虫学]